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- PDB-2o3w: Crystal Structure of the Homo sapiens Cytoplasmic Ribosomal Decod... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2o3w | ||||||||||||||||||
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Title | Crystal Structure of the Homo sapiens Cytoplasmic Ribosomal Decoding Site in presence of paromomycin | ||||||||||||||||||
![]() | RNA (5'-R(*![]() RNA / aminoglycoside / antibiotics / ribosome / decoding site / Homo sapiens / Eukaryote / cytoplasmic / Translation inhibition / Stop codon readthrough / unspecific binding | Function / homology | PAROMOMYCIN / RNA / RNA (> 10) | ![]() Method | ![]() ![]() ![]() ![]() Kondo, J. / Hainrichson, M. / Nudelman, I. / Shallom-Shezifi, D. / Baasov, T. / Westhof, E. | ![]() ![]() Title: Differential Selectivity of Natural and Synthetic Aminoglycosides towards the Eukaryotic and Prokaryotic Decoding A Sites. Authors: Kondo, J. / Hainrichson, M. / Nudelman, I. / Shallom-Shezifi, D. / Barbieri, C.M. / Pilch, D.S. / Westhof, E. / Baasov, T. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 34.3 KB | Display | ![]() |
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PDB format | ![]() | 24.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 716.3 KB | Display | ![]() |
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Full document | ![]() | 718.2 KB | Display | |
Data in XML | ![]() | 4.6 KB | Display | |
Data in CIF | ![]() | 5.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2o3vC ![]() 2o3xC ![]() 2o3yC ![]() 2fqnS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: RNA chain | Mass: 7355.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: Chemically synthesized #2: Chemical | ChemComp-PAR / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.27 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 300 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: Sodium Cacodylate, Potassium chloride, 2-methyl-2,4-pentanediol, hexammine cobalt chloride, spermine tetrahydrochloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 28, 2005 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.8→57.397 Å / Num. obs: 3672 / % possible obs: 99.8 % / Redundancy: 6.2 % / Rmerge(I) obs: 0.085 / Rsym value: 0.085 / Net I/σ(I): 5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2FQN Resolution: 2.8→57.35 Å / FOM work R set: 0.725 / σ(F): 3 Stereochemistry target values: G. Parkinson, J. Vojtechovsky, L. Clowney, A.T. Brunger, H.M. Berman, New Parameters for the Refinement of Nucleic Acid Containing Structures, Acta Cryst. D, 52, 57-64 (1996).
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Solvent computation | Bsol: 125.091 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 72.414 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→57.35 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 8
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Xplor file |
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