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- PDB-2nyy: Crystal structure of botulinum neurotoxin type A complexed with m... -

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Basic information

Entry
Database: PDB / ID: 2nyy
TitleCrystal structure of botulinum neurotoxin type A complexed with monoclonal antibody CR1
Components
  • (CR1 monoclonal antibody) x 2
  • Botulinum neurotoxin type A
KeywordsTOXIN/IMMUNE SYSTEM / BOTULINUM / NEUROTOXIN / FAB / PROTEIN ANTIBODY COMPLEX / TOXIN-IMMUNE SYSTEM COMPLEX
Function / homology
Function and homology information


host cell junction / negative regulation of neurotransmitter secretion / bontoxilysin / host cell presynaptic membrane / host cell cytoplasmic vesicle / host cell cytosol / protein transmembrane transporter activity / membrane => GO:0016020 / metalloendopeptidase activity / toxin activity ...host cell junction / negative regulation of neurotransmitter secretion / bontoxilysin / host cell presynaptic membrane / host cell cytoplasmic vesicle / host cell cytosol / protein transmembrane transporter activity / membrane => GO:0016020 / metalloendopeptidase activity / toxin activity / host cell plasma membrane / proteolysis / zinc ion binding / extracellular region / membrane
Similarity search - Function
Clostridium botulinum neurotoxin B, "coiled-coil" domain / Clostridium botulinum neurotoxin b, "coiled-coil" domain / Zincin-like / Metalloproteases ("zincins"), catalytic domain like / Clostridium neurotoxin, translocation / Clostridium neurotoxin, Translocation domain / Clostridium neurotoxin, translocation domain / Clostridial neurotoxin zinc protease / Botulinum/Tetanus toxin, catalytic chain / Clostridium neurotoxin, receptor-binding C-terminal ...Clostridium botulinum neurotoxin B, "coiled-coil" domain / Clostridium botulinum neurotoxin b, "coiled-coil" domain / Zincin-like / Metalloproteases ("zincins"), catalytic domain like / Clostridium neurotoxin, translocation / Clostridium neurotoxin, Translocation domain / Clostridium neurotoxin, translocation domain / Clostridial neurotoxin zinc protease / Botulinum/Tetanus toxin, catalytic chain / Clostridium neurotoxin, receptor-binding C-terminal / Clostridium neurotoxin, receptor binding N-terminal / Clostridium neurotoxin, C-terminal receptor binding / Clostridium neurotoxin, N-terminal receptor binding / Kunitz inhibitor STI-like superfamily / Trefoil (Acidic Fibroblast Growth Factor, subunit A) - #50 / Trefoil (Acidic Fibroblast Growth Factor, subunit A) / Trefoil / Jelly Rolls - #200 / Neutral zinc metallopeptidases, zinc-binding region signature. / Concanavalin A-like lectin/glucanase domain superfamily / Jelly Rolls / Immunoglobulins / Alpha-Beta Complex / Up-down Bundle / Immunoglobulin-like / Sandwich / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
Botulinum neurotoxin type A / Botulinum neurotoxin type A
Similarity search - Component
Biological speciesHomo sapiens (human)
Clostridium botulinum (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.61 Å
AuthorsStevens, R.C. / Arndt, J.W.
CitationJournal: Nat.Biotechnol. / Year: 2007
Title: Molecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxin.
Authors: Garcia-Rodriguez, C. / Levy, R. / Arndt, J.W. / Forsyth, C.M. / Razai, A. / Lou, J. / Geren, I. / Stevens, R.C. / Marks, J.D.
History
DepositionNov 21, 2006Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 26, 2006Provider: repository / Type: Initial release
Revision 1.1May 1, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Advisory / Version format compliance
Revision 1.3Feb 17, 2016Group: Derived calculations
Revision 1.4Aug 30, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id
Revision 1.5Nov 6, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature
Remark 999SEQUENCE There is no aminoacid sequence database reference available for the CR1 monoclonal ...SEQUENCE There is no aminoacid sequence database reference available for the CR1 monoclonal antibody light and heavy chains

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Botulinum neurotoxin type A
C: CR1 monoclonal antibody
D: CR1 monoclonal antibody
hetero molecules


Theoretical massNumber of molelcules
Total (without water)197,5355
Polymers197,4303
Non-polymers1052
Water00
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5710 Å2
ΔGint-70 kcal/mol
Surface area70030 Å2
MethodPISA
Unit cell
Length a, b, c (Å)98.046, 148.249, 196.250
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein Botulinum neurotoxin type A


Mass: 149479.812 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Clostridium botulinum (bacteria) / Strain: Type A1 (HALL STRAIN)
References: UniProt: P10845, UniProt: P0DPI1*PLUS, bontoxilysin
#2: Antibody CR1 monoclonal antibody


Mass: 23896.471 Da / Num. of mol.: 1 / Fragment: light chain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): CHO DG44 CELLS / Production host: Cricetulus griseus (Chinese hamster)
#3: Antibody CR1 monoclonal antibody


Mass: 24053.721 Da / Num. of mol.: 1 / Fragment: heavy chain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): CHO DG44 CELLS / Production host: Cricetulus griseus (Chinese hamster)
#4: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn
#5: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Ca
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.61 Å3/Da / Density % sol: 65.94 %
Crystal growTemperature: 297 K / Method: vapor diffusion, sitting drop / pH: 5.5
Details: 8% PEG 20000, 8% PEG 550 MME, 200 mM calcium acetate, 100 mM sodium acetate, pH 5.50, VAPOR DIFFUSION, SITTING DROP, temperature 297K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL11-1 / Wavelength: 1
DetectorType: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Feb 1, 2005
RadiationMonochromator: DOUBLE CRYSTAL, PARALELL (SI 111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.6→50 Å / Num. obs: 87498 / % possible obs: 89.4 % / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.095
Reflection shellResolution: 2.61→2.68 Å / Rmerge(I) obs: 0.388 / % possible all: 44.8

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Processing

Software
NameVersionClassification
REFMAC5.2.0019refinement
HKL-2000data reduction
HKL-2000data scaling
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 3BTA

3bta


Resolution: 2.61→50 Å / Cor.coef. Fo:Fc: 0.945 / Cor.coef. Fo:Fc free: 0.922 / SU B: 28.209 / SU ML: 0.256 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / σ(F): 2 / ESU R: 0.461 / ESU R Free: 0.289 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.246 3912 5 %RANDOM
Rwork0.206 ---
obs0.208 74507 89.4 %-
all-87498 --
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK
Displacement parametersBiso mean: 11.41 Å2
Baniso -1Baniso -2Baniso -3
1--4.77 Å20 Å20 Å2
2--0.04 Å20 Å2
3---4.73 Å2
Refinement stepCycle: LAST / Resolution: 2.61→50 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13505 0 2 0 13507
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0130.02213807
X-RAY DIFFRACTIONr_bond_other_d0.0020.029244
X-RAY DIFFRACTIONr_angle_refined_deg1.51.9518717
X-RAY DIFFRACTIONr_angle_other_deg0.9083.00122545
X-RAY DIFFRACTIONr_dihedral_angle_1_deg11.26451694
X-RAY DIFFRACTIONr_dihedral_angle_2_deg38.12324.962661
X-RAY DIFFRACTIONr_dihedral_angle_3_deg17.83152348
X-RAY DIFFRACTIONr_dihedral_angle_4_deg16.4941557
X-RAY DIFFRACTIONr_chiral_restr0.0850.22054
X-RAY DIFFRACTIONr_gen_planes_refined0.0040.0215424
X-RAY DIFFRACTIONr_gen_planes_other0.0010.022812
X-RAY DIFFRACTIONr_nbd_refined0.2290.23019
X-RAY DIFFRACTIONr_nbd_other0.2060.29500
X-RAY DIFFRACTIONr_nbtor_refined0.1970.26822
X-RAY DIFFRACTIONr_nbtor_other0.0910.27686
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1650.2278
X-RAY DIFFRACTIONr_xyhbond_nbd_other0.0270.23
X-RAY DIFFRACTIONr_metal_ion_refined0.1340.23
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.220.28
X-RAY DIFFRACTIONr_symmetry_vdw_other0.2240.226
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.2060.25
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it0.5371.58626
X-RAY DIFFRACTIONr_mcbond_other0.0781.53436
X-RAY DIFFRACTIONr_mcangle_it0.907213654
X-RAY DIFFRACTIONr_scbond_it1.20935924
X-RAY DIFFRACTIONr_scangle_it1.9684.55063
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 2.61→2.68 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.384 143 -
Rwork0.389 2733 -
obs--44.81 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.84151.3061-3.37250.4845-1.34083.7552-0.11450.0482-0.34010.3123-0.35680.12160.4488-0.13750.47120.2106-0.13450.242-0.1701-0.13990.006-62.8574-44.715757.8033
28.1381-8.5304-1.856144.773323.690914.2188-0.7019-0.5780.4191.32430.1744-0.34090.3086-1.05890.5275-0.0162-0.05690.1407-0.0819-0.1817-0.0652-84.2427-26.946591.682
37.6265-0.2318-9.319427.71356.691712.8702-0.1525-0.2064-0.84770.80190.01160.287-0.6633-0.0920.1409-0.00630.00240.1555-0.0766-0.2567-0.0893-79.2179-45.94280.0905
42.6848-1.0963-2.485111.64373.53175.07690.0987-0.2146-0.1408-0.1404-0.0850.7909-0.2962-0.4425-0.0137-0.1346-0.15250.18680.0693-0.207-0.0373-88.0001-38.083683.1678
54.0705-0.4104-2.85693.25791.27925.65740.22550.00480.32530.6841-0.17560.1816-0.31820.2254-0.04990.0557-0.09160.1426-0.22950.0115-0.0531-54.2048-22.445558.627
644.6618-16.8033.097415.0196-3.68040.94210.90232.6209-0.2302-0.4988-0.7790.2232-0.70080.2509-0.12340.1662-0.05310.24970.0833-0.06780.0632-59.7603-15.336559.0147
710.479-4.5367-6.57636.50064.78817.6524-0.1278-0.32580.40860.93670.03630.14790.57710.36430.0915-0.0172-0.13830.1066-0.1740.0026-0.082-54.5023-30.847158.8154
81.9039-1.195-0.51926.59940.24876.42680.3638-0.35710.36490.76830.38990.7933-0.3277-0.1219-0.7537-0.1071-0.1060.0922-0.0061-0.3520.0806-73.6265-28.807384.8943
94.4798-1.1356-0.68316.7836-2.28685.50640.0288-0.52050.13770.31770.3266-0.4331-0.22050.3247-0.35540.015-0.15020.23-0.16-0.4063-0.0665-70.4296-30.084786.8133
109.7397-0.23671.13735.5732-0.03755.62890.03720.41270.1756-0.2995-0.0978-0.4483-0.05110.50390.0606-0.2691-0.06490.04420.01640.2372-0.156341.7694-8.33439.1067
115.48622.2951-0.67314.3596-3.32696.1495-0.0152-0.5490.84020.2437-0.32960.0205-0.39760.31270.3447-0.3347-0.0219-0.0374-0.00150.0779-0.107137.4766-16.820727.8546
123.88980.0994-0.22312.8171-0.16871.7674-0.0010.41360.5004-0.3633-0.0438-0.2535-0.45730.30130.0448-0.1211-0.01270.006-0.06830.2159-0.23232.7679-3.18829.2113
137.1968-10.184314.302322.002-19.522228.4910.6655-0.86141.35250.99870.44310.9286-0.2658-0.6548-1.1086-0.3272-0.02520.0851-0.14350.0924-0.041331.8111-16.080124.8093
143.037-1.0135-0.10232.6256-0.38021.85020.1104-0.1815-0.11820.2476-0.06150.1144-0.2107-0.0555-0.0489-0.2807-0.0374-0.0069-0.0880.0918-0.232921.6363-12.106720.1081
152.84670.9128-1.47692.9977-2.0582.62630.1875-0.27350.13050.275-0.2985-0.0639-0.6154-0.04790.111-0.10030.0377-0.0172-0.06260.0768-0.073413.9563-0.289521.9239
161.60890.6551-0.50469.2861-1.60911.39530.05220.62150.1879-0.60020.05210.3438-0.4308-0.336-0.10430.02910.0864-0.0391-0.03130.1811-0.222815.79482.90756.7631
173.4864-0.4355-1.49951.4781-0.8427.64960.08740.022-0.5533-0.19890.09880.24040.2926-0.246-0.1862-0.3082-0.0363-0.0183-0.19720.1131-0.007522.3611-28.823720.9991
187.2287-5.96445.260917.441-9.16188.0852-0.1423-0.4574-0.61130.30410.04830.5037-0.364-0.02150.094-0.416-0.03980.04130.05160.1812-0.038711.5867-20.578339.516
1911.4704-8.86778.94647.8824-8.416310.92450.45750.59970.2833-0.3011-0.3435-0.4639-0.38710.7959-0.1140.04730.00170.2035-0.03620.03450.1636-7.52795.437321.1125
2016.2697-1.0038-4.222711.4969-1.942316.22690.23280.47080.0861-0.36990.01910.339-0.9465-1.0625-0.2519-0.21120.0091-0.162-0.1006-0.0059-0.111610.1408-8.76291.5652
213.3479-1.7249-0.36436.4664-2.15958.97350.14580.14630.6954-0.0995-0.303-0.1097-0.0862-0.70120.1572-0.1302-0.19510.0203-0.056-0.00080.052134.35113.268915.5869
2212.61285.1231.97149.13022.89093.1996-0.153-1.1085-0.2517-0.09540.43820.4272-0.5875-0.129-0.2852-0.1218-0.06110.02650.13940.3920.045612.8096-25.715645.6727
237.6976-0.48352.61629.03593.32926.81650.0262-0.5151-1.04850.8544-0.2695-0.18880.86440.31780.2433-0.0322-0.06760.1647-0.15260.40760.71949.4293-50.8936.9124
242.373-1.2156-0.02486.6126-0.68340.80790.1686-0.1476-0.7450.2709-0.07420.0854-0.0310.0153-0.0944-0.3809-0.0591-0.0018-0.06070.1310.1288-9.0666-18.76131.0341
252.8719-1.99010.18395.2758-1.03891.13160.19010.2299-0.6373-0.5153-0.1284-0.02120.0409-0.0897-0.0617-0.3-0.00440.0135-0.1199-0.030.0964-5.6774-19.648222.2473
263.61452.7538-2.82318.10411.39364.29690.2148-0.1516-0.7135-0.0095-0.532-0.54160.35280.82210.3171-0.3752-0.0352-0.06010.1250.09290.1355-21.6547-28.292233.7581
274.1795-0.91211.94770.885-1.33356.18740.068-0.0866-0.21350.77970.0924-1.1118-0.38330.5187-0.1604-0.2283-0.0392-0.03490.03630.1602-0.2161-26.6664-28.329140.9205
2810.2389-4.4574-3.82364.50763.00317.03710.3343-0.44060.29270.1098-0.1282-0.1329-0.00550.5153-0.206-0.2911-0.0406-0.0332-0.09010.1613-0.1495-35.3053-31.390541.5066
294.87291.0747-0.95281.3148-0.05661.04820.07630.15730.46870.2321-0.08530.1478-0.2355-0.01750.009-0.34120.0171-0.0112-0.08540.129-0.1555-37.0691-22.729231.6815
308.40695.78840.23287.9816-2.44662.6707-0.1027-0.28511.03540.1653-0.28280.2268-0.8017-0.12120.3855-0.2560.01870.0076-0.05550.1218-0.0758-41.0983-17.497630.5817
313.81341.35190.11842.7969-2.7493.8398-0.1930.46850.69820.14980.14920.1452-0.33070.11610.0438-0.25790.02440.0004-0.02890.1375-0.1805-40.919-22.566821.7623
321.32340.14-0.10133.0415-0.04311.2742-0.09490.1947-0.0660.00390.0142-0.08660.36710.09020.0807-0.2293-0.00460.0375-0.03630.0908-0.2743-39.8062-44.191527.109
334.20980.5888-0.6036.851.66336.38440.16080.2773-0.2529-0.0134-0.0128-0.29870.8660.2249-0.148-0.11530.12190.0688-0.1670.1121-0.1595-32.8475-62.416221.3251
341.0067-2.7708-1.19867.72542.055917.04220.22160.67490.2112-0.55370.1389-0.3006-0.37320.5905-0.3604-0.1668-0.02020.11790.01470.03-0.2063-34.6349-55.73759.574
3517.7215-6.51040.574715.24743.19343.9106-0.0396-0.49650.10950.3529-0.08410.28940.6936-0.42870.1237-0.236-0.04460.0628-0.18780.1556-0.3761-45.6414-47.539732.4167
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
1X-RAY DIFFRACTION1CB1 - 1201 - 120
2X-RAY DIFFRACTION2CB121 - 137121 - 137
3X-RAY DIFFRACTION3CB138 - 145138 - 145
4X-RAY DIFFRACTION4CB146 - 216146 - 216
5X-RAY DIFFRACTION5DC2 - 782 - 78
6X-RAY DIFFRACTION6DC79 - 8779 - 87
7X-RAY DIFFRACTION7DC88 - 10988 - 109
8X-RAY DIFFRACTION8DC110 - 145110 - 145
9X-RAY DIFFRACTION9DC146 - 218146 - 218
10X-RAY DIFFRACTION10AA2 - 441 - 43
11X-RAY DIFFRACTION11AA45 - 8244 - 81
12X-RAY DIFFRACTION12AA83 - 15382 - 152
13X-RAY DIFFRACTION13AA154 - 163153 - 162
14X-RAY DIFFRACTION14AA164 - 243163 - 242
15X-RAY DIFFRACTION15AA244 - 308243 - 307
16X-RAY DIFFRACTION16AA309 - 357308 - 356
17X-RAY DIFFRACTION17AA358 - 431357 - 430
18X-RAY DIFFRACTION18AA453 - 469452 - 468
19X-RAY DIFFRACTION19AA470 - 488469 - 487
20X-RAY DIFFRACTION20AA493 - 507492 - 506
21X-RAY DIFFRACTION21AA508 - 533507 - 532
22X-RAY DIFFRACTION22AA534 - 556533 - 555
23X-RAY DIFFRACTION23AA557 - 621556 - 620
24X-RAY DIFFRACTION24AA627 - 688626 - 687
25X-RAY DIFFRACTION25AA689 - 872688 - 871
26X-RAY DIFFRACTION26AA873 - 890872 - 889
27X-RAY DIFFRACTION27AA891 - 908890 - 907
28X-RAY DIFFRACTION28AA909 - 923908 - 922
29X-RAY DIFFRACTION29AA924 - 962923 - 961
30X-RAY DIFFRACTION30AA963 - 989962 - 988
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