分子量: 8970.373 Da / 分子数: 1 / 由来タイプ: 合成 / 詳細: Transcription of RNA using T7 polymerase
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D 1H-13C HSQC
1
2
1
3D (H)CCH-TOCSY
1
3
1
2DNOESY13CHSQC
1
4
1
2DHCNTROSY
1
5
1
2D NOESY
1
6
3
2D 1H-13C HSQC
2
7
2
2D NOESY
2
8
2
2D COSY
1
9
2
2D 1H-13C HSQC
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試料調製
詳細
Solution-ID
内容
溶媒系
1
1.5 mM [U-98% 13C; U-98% 15N] ADENOSINE-5'-MONOPHOSPHATE, 1.5 mM [U-98% 13C; U-98% 15N] URIDINE-5'-MONOPHOSPHATE, 1.5 mM GUANOSINE-5'-MONOPHOSPHATE, 1.5 mM CYTIDINE-5'-MONOPHOSPHATE, 1.5 mM cobalt hexamine, 10 mM sodium chloride, 10 mM sodium phosphate, 100% D2O
100% D2O
2
1 mM GUANOSINE-5'-MONOPHOSPHATE, 1 mM ADENOSINE-5'-MONOPHOSPHATE, 1 mM CYTIDINE-5'-MONOPHOSPHATE, 1 mM URIDINE-5'-MONOPHOSPHATE, 1 mM cobalt hexamine, 10 mM sodium chloride, 10 mM sodium phosphate, 90% H2O/10% D2O
90% H2O/10% D2O
3
1.5 mM [U-98% 13C; U-98% 15N] ADENOSINE-5'-MONOPHOSPHATE, 1.5 mM [U-98% 13C; U-98% 15N] URIDINE-5'-MONOPHOSPHATE, 1.5 mM GUANOSINE-5'-MONOPHOSPHATE, 1.5 mM CYTIDINE-5'-MONOPHOSPHATE, 1.5 mM cobalt hexamine, 5 mg/mL phage, 10 mM sodium chloride, 10 mM sodium phosphate, 100% D2O
100% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
1.5mM
ADENOSINE-5'-MONOPHOSPHATE-1
[U-98% 13C; U-98% 15N]
1
1.5mM
URIDINE-5'-MONOPHOSPHATE-2
[U-98% 13C; U-98% 15N]
1
1.5mM
GUANOSINE-5'-MONOPHOSPHATE-3
1
1.5mM
CYTIDINE-5'-MONOPHOSPHATE-4
1
1.5mM
cobalt hexamine-5
1
10mM
sodium chloride-6
1
10mM
sodium phosphate-7
1
1mM
GUANOSINE-5'-MONOPHOSPHATE-8
2
1mM
ADENOSINE-5'-MONOPHOSPHATE-9
2
1mM
CYTIDINE-5'-MONOPHOSPHATE-10
2
1mM
URIDINE-5'-MONOPHOSPHATE-11
2
1mM
cobalt hexamine-12
2
10mM
sodium chloride-13
2
10mM
sodium phosphate-14
2
1.5mM
ADENOSINE-5'-MONOPHOSPHATE-15
[U-98% 13C; U-98% 15N]
3
1.5mM
URIDINE-5'-MONOPHOSPHATE-16
[U-98% 13C; U-98% 15N]
3
1.5mM
GUANOSINE-5'-MONOPHOSPHATE-17
3
1.5mM
CYTIDINE-5'-MONOPHOSPHATE-18
3
1.5mM
cobalt hexamine-19
3
5mg/mL
phage-20
3
10mM
sodium chloride-21
3
10mM
sodium phosphate-22
3
試料状態
Conditions-ID
イオン強度
pH
圧 (kPa)
温度 (K)
1
20
6
ambient
298K
2
20
6
ambient
274K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Varian VNMRS
Varian
VNMRS
500
1
Agilent INOVA
Agilent
INOVA
900
2
Agilent INOVA
Agilent
INOVA
800
3
Agilent NMR System
Agilent
NMRSystem
600
4
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解析
NMR software
名称
開発者
分類
Sparky
Goddard
chemicalshiftassignment
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 10