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Yorodumi- PDB-2nbs: Backbone 1H, 13C, and 15N Chemical Shift Assignments for designed... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2nbs | ||||||
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Title | Backbone 1H, 13C, and 15N Chemical Shift Assignments for designed protein E_1r26 | ||||||
Components | E_1r26 | ||||||
Keywords | UNKNOWN FUNCTION / designed protein | ||||||
Function / homology | Glutaredoxin / Glutaredoxin / 3-Layer(aba) Sandwich / Alpha Beta Function and homology information | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Zhou, X. | ||||||
Citation | Journal: To be Published Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for designed protein E_1r26 Authors: Zhou, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2nbs.cif.gz | 837.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2nbs.ent.gz | 713.1 KB | Display | PDB format |
PDBx/mmJSON format | 2nbs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2nbs_validation.pdf.gz | 469.2 KB | Display | wwPDB validaton report |
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Full document | 2nbs_full_validation.pdf.gz | 802.3 KB | Display | |
Data in XML | 2nbs_validation.xml.gz | 88.6 KB | Display | |
Data in CIF | 2nbs_validation.cif.gz | 80 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nb/2nbs ftp://data.pdbj.org/pub/pdb/validation_reports/nb/2nbs | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13990.840 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.02mM sodium phosphate-1, 0.01mM EDTA-2, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | |||||||||
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Sample |
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Sample conditions | Ionic strength: 0.05 / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 1000 / Conformers submitted total number: 20 / Representative conformer: 1 |