Generic Transcription Pathway / : / DNA-binding transcription factor activity, RNA polymerase II-specific / nuclear body / RNA polymerase II cis-regulatory region sequence-specific DNA binding / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / DNA binding / nucleus / metal ion binding 類似検索 - 分子機能
0.8 mM [U-99% 15N] sodium phosphate, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.8mMsodiumphosphate, 90% H2O/10% D2O
90% H2O/10% D2O
4
0.8mMsodiumphosphate, 100% D2O
100% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
0.8mM
sodium phosphate-1
[U-99% 13C; U-99% 15N]
1
0.8mM
sodium phosphate-2
[U-99% 15N]
2
0.8mM
sodium phosphate-3
3
0.8mM
sodium phosphate-4
4
試料状態
イオン強度: 0.15 / pH: 6.3 / 圧: ambient / 温度: 295 K
-
NMR測定
NMRスペクトロメーター
タイプ: Bruker Avance / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 600 MHz
-
解析
NMR software
名称
開発者
分類
ARIA
Linge, O'DonoghueandNilges
構造決定
ARIA
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1
NMR constraints
NOE constraints total: 569 / NOE intraresidue total count: 191 / NOE long range total count: 100 / NOE medium range total count: 31 / NOE sequential total count: 156 / Protein phi angle constraints total count: 20 / Protein psi angle constraints total count: 20
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 15