[English] 日本語
Yorodumi- PDB-2n8q: NMR solution structure of S114A mutant of a UV inducible protein ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2n8q | ||||||
---|---|---|---|---|---|---|---|
Title | NMR solution structure of S114A mutant of a UV inducible protein from Chlamydomonas reinhardtii | ||||||
Components | Predicted protein | ||||||
Keywords | HYDROLASE INHIBITOR / DNA HYDROLASE Deactivator | ||||||
Function / homology | BolA-like / BolA protein / BolA-like superfamily / BolA-like protein / GMP Synthetase; Chain A, domain 3 / 2-Layer Sandwich / Alpha Beta / Predicted protein Function and homology information | ||||||
Biological species | Chlamydomonas reinhardtii (plant) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 22 | ||||||
Authors | Singh, H. | ||||||
Citation | Journal: To be Published Title: NMR solution structure of S114A mutant of a UV inducible protein from Chlamydomonas reinhardtii Authors: Singh, H. / Chary, K. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2n8q.cif.gz | 915.1 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2n8q.ent.gz | 791 KB | Display | PDB format |
PDBx/mmJSON format | 2n8q.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2n8q_validation.pdf.gz | 404.2 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 2n8q_full_validation.pdf.gz | 578.6 KB | Display | |
Data in XML | 2n8q_validation.xml.gz | 43.5 KB | Display | |
Data in CIF | 2n8q_validation.cif.gz | 67.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n8/2n8q ftp://data.pdbj.org/pub/pdb/validation_reports/n8/2n8q | HTTPS FTP |
-Related structure data
Similar structure data | |
---|---|
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 13312.992 Da / Num. of mol.: 1 / Mutation: S114A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chlamydomonas reinhardtii (plant) / Strain: cc125 / Gene: CHLREDRAFT_180018 / Production host: Escherichia coli (E. coli) / References: UniProt: A8JH36 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR Details: NMR solution structure of S114A mutant of a UV inducible protein from Chlamydomonas reinhardtii | ||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||||||||||||||||||||||||||
NMR details | Text: Marie Sklodowska-Curie Individual Fellowship (IF) |
-Sample preparation
Details | Contents: 1 mM [U-100% 13C; U-100% 15N] S114A-1, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
---|---|
Sample | Conc.: 1 mM / Component: S114A-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 0.1 / pH: 6.4 / Pressure: ambient / Temperature: 25 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
---|
-Processing
NMR software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 22 / Representative conformer: 22 |