+Open data
-Basic information
Entry | Database: PDB / ID: 2n8o | ||||||
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Title | NMR Solution Structure of Aureocin A53 | ||||||
Components | Bacteriocin aureocin A53 | ||||||
Keywords | ANTIMICROBIAL PROTEIN / bacteriocin / leaderless bacteriocin | ||||||
Function / homology | Bacteriocin class II, aureocin-like / Aureocin-like type II bacteriocin / killing of cells of another organism / defense response to bacterium / extracellular region / Bacteriocin aureocin A53 Function and homology information | ||||||
Biological species | Staphylococcus aureus (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Acedo, J.Z. / van Belkum, M.J. / Lohans, C.T. / Towle, K.M. / Miskolzie, M. / Vederas, J.C. | ||||||
Citation | Journal: Biochemistry / Year: 2016 Title: Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity. Authors: Acedo, J.Z. / van Belkum, M.J. / Lohans, C.T. / Towle, K.M. / Miskolzie, M. / Vederas, J.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2n8o.cif.gz | 393.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2n8o.ent.gz | 335.2 KB | Display | PDB format |
PDBx/mmJSON format | 2n8o.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n8/2n8o ftp://data.pdbj.org/pub/pdb/validation_reports/n8/2n8o | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5994.228 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: aucA / Plasmid: pET-SUMO / Production host: Escherichia coli (E. coli) / References: UniProt: Q8GPI4 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1 mM Aureocin A53, 0.0001 w/v DSS, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | |||||||||
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Sample |
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Sample conditions | Ionic strength: 0 / pH: 7 / Pressure: ambient / Temperature: 300 K |
-NMR measurement
NMR spectrometer | Type: Varian vNMRJ / Manufacturer: Varian / Model: vNMRJ / Field strength: 700 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |