詳細 | Solution-ID | 内容 | 溶媒系 |
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1 | 0.6 mM [U-13C; U-15N]-Gua, -Ura RNA (92-MER), 0.02 mM potassium chloride, 0.02 mM potassium phosphate, 95% H2O/5% D2O | 95% H2O/5% D2O2 | 0.7 mM [U-13C; U-15N]-Ade RNA (92-MER), 0.02 mM potassium chloride, 0.02 mM potassium phosphate, 95% H2O/5% D2O | 95% H2O/5% D2O3 | 0.6 mM [U-13C; U-15N]-Gua, -Ura RNA (92-MER), 0.02 mM potassium chloride, 0.02 mM potassium phosphate, 5 mg/mL Pf1 phage, 95% H2O/5% D2O | 95% H2O/5% D2O4 | 0.7 mM [U-13C; U-15N]-Ade RNA (92-MER), 0.02 mM potassium chloride, 0.02 mM potassium phosphate, 5 mg/mL Pf1 phage, 95% H2O/5% D2O | 95% H2O/5% D2O | | | |
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試料 | 濃度 (mg/ml) | 構成要素 | Isotopic labeling | Solution-ID |
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0.6 mMRNA (92-MER)-1 | [U-13C; U-15N]-Gua, -Ura | 1 | 0.02 mMpotassium chloride-2 | | 1 | 0.02 mMpotassium phosphate-3 | | 1 | 0.7 mMRNA (92-MER)-4 | [U-13C; U-15N]-Ade | 2 | 0.02 mMpotassium chloride-5 | | 2 | 0.02 mMpotassium phosphate-6 | | 2 | 0.6 mMRNA (92-MER)-7 | [U-13C; U-15N]-Gua, -Ura | 3 | 0.02 mMpotassium chloride-8 | | 3 | 0.02 mMpotassium phosphate-9 | | 3 | 5 mg/mLPf1 phage-10 | | 3 | 0.7 mMRNA (92-MER)-11 | [U-13C; U-15N]-Ade | 4 | 0.02 mMpotassium chloride-12 | | 4 | 0.02 mMpotassium phosphate-13 | | 4 | 5 mg/mLPf1 phage-14 | | 4 | | | | | | | | | | | | | | |
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試料状態 | Conditions-ID | イオン強度 | pH | 圧 (kPa) | 温度 (K) |
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1 | 58.5 | 7 | ambient 298 K2 | 58.5 | 7 | ambient 303 K | | | |
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