1 mM [U-13C; U-15N] RNA polymerase II transcription factor B subunit 1, 2 mM Non-structural protein NS-S, 90% H2O/10% D2O
90% H2O/10% D2O
2
1 mM [U-13C; U-15N] RNA polymerase II transcription factor B subunit 1, 2 mM Non-structural protein NS-S, 100% D2O
100% D2O
3
0.5 mM [U-15N] RNA polymerase II transcription factor B subunit 1, 1 mM Non-structural protein NS-S, 90% H2O/10% D2O
90% H2O/10% D2O
4
1 mM RNA polymerase II transcription factor B subunit 1, 0.5 mM [U-13C; U-15N] Non-structural protein NS-S, 90% H2O/10% D2O
90% H2O/10% D2O
5
0.5 mM RNA polymerase II transcription factor B subunit 1, 1 mM [U-13C; U-15N] Non-structural protein NS-S, 100% D2O
100% D2O
6
0.4 mM RNA polymerase II transcription factor B subunit 1, 0.2 mM [U-15N] Non-structural protein NS-S, 90% H2O/10% D2O
90% H2O/10% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
1mM
RNA polymerase II transcription factor B subunit 1-1
[U-13C; U-15N]
1
2mM
Non-structural protein NS-S-2
1
1mM
RNA polymerase II transcription factor B subunit 1-3
[U-13C; U-15N]
2
2mM
Non-structural protein NS-S-4
2
0.5mM
RNA polymerase II transcription factor B subunit 1-5
[U-15N]
3
1mM
Non-structural protein NS-S-6
3
1mM
RNA polymerase II transcription factor B subunit 1-7
4
0.5mM
Non-structural protein NS-S-8
[U-13C; U-15N]
4
0.5mM
RNA polymerase II transcription factor B subunit 1-9
5
1mM
Non-structural protein NS-S-10
[U-13C; U-15N]
5
0.4mM
RNA polymerase II transcription factor B subunit 1-11
6
0.2mM
Non-structural protein NS-S-12
[U-15N]
6
試料状態
イオン強度: 0 / pH: 6.5 / 圧: ambient / 温度: 300 K
-
NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
500
1
Varian INOVA
Varian
INOVA
600
2
-
解析
NMR software
名称
バージョン
分類
CcpNmr Analysis
2.3.1
データ解析
CNS
精密化
精密化
手法: torsion angle dynamics / ソフトェア番号: 1
NMR constraints
NOE constraints total: 3053 / NOE intraresidue total count: 1190 / NOE long range total count: 794 / NOE medium range total count: 417 / NOE sequential total count: 622 / Protein phi angle constraints total count: 111 / Protein psi angle constraints total count: 111
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 260 / 登録したコンフォーマーの数: 20