- PDB-2mxb: Structure of the transmembrane domain of the mouse erythropoietin... -
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基本情報
登録情報
データベース: PDB / ID: 2mxb
タイトル
Structure of the transmembrane domain of the mouse erythropoietin receptor
要素
Erythropoietin receptor
キーワード
MEMBRANE PROTEIN
機能・相同性
機能・相同性情報
: / Erythropoietin activates RAS / erythropoietin receptor activity / visceral serous pericardium development / vasculogenesis involved in coronary vascular morphogenesis / Erythropoietin activates Phosphoinositide-3-kinase (PI3K) / negative regulation of nitric oxide biosynthetic process / cardiac muscle tissue morphogenesis / ventricular cardiac muscle tissue morphogenesis / decidualization ...: / Erythropoietin activates RAS / erythropoietin receptor activity / visceral serous pericardium development / vasculogenesis involved in coronary vascular morphogenesis / Erythropoietin activates Phosphoinositide-3-kinase (PI3K) / negative regulation of nitric oxide biosynthetic process / cardiac muscle tissue morphogenesis / ventricular cardiac muscle tissue morphogenesis / decidualization / heart morphogenesis / viral process / brain development / positive regulation of neuron projection development / heart development / positive regulation of cytosolic calcium ion concentration / negative regulation of neuron apoptotic process / membrane => GO:0016020 / positive regulation of ERK1 and ERK2 cascade / nuclear speck / external side of plasma membrane / positive regulation of cell population proliferation / signal transduction / extracellular region / identical protein binding / plasma membrane / cytoplasm 類似検索 - 分子機能
Erythropoietin receptor / Long hematopoietin receptor, single chain, conserved site / Long hematopoietin receptor, single chain family signature. / Growth hormone/erythropoietin receptor, ligand binding / Erythropoietin receptor, ligand binding / Fibronectin type III domain / Fibronectin type 3 domain / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Immunoglobulin-like fold 類似検索 - ドメイン・相同性
0.5-1 mM [U-100% 15N] protein, 20 mM potassium phosphate, 240 mM [U-100% 15N] DPC, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.8 mM [U-100% 13C; U-100% 15N] protein, 20 mM sodium phosphate, 240 mM [U-2H] DPC, 90% H2O/10% D2O
90% H2O/10% D2O
試料
濃度 (mg/ml)
単位
構成要素
Isotopic labeling
Conc. range (mg/ml)
Solution-ID
mM
entity-1
[U-100% 15N]
0.5-1
1
20mM
potassium phosphate-2
1
240mM
DPC-3
[U-100% 15N]
1
0.8mM
entity-4
[U-100% 13C; U-100% 15N]
2
20mM
sodium phosphate-5
2
240mM
DPC-6
[U-2H]
2
試料状態
イオン強度: 20 / pH: 6.5 / 圧: ambient / 温度: 313 K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker Avance
Bruker
AVANCE
700
1
Bruker Avance
Bruker
AVANCE
600
2
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解析
NMR software
名称
開発者
分類
NMRView
Johnson, OneMoonScientific
peakpicking
NMRPipe
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
構造決定
TopSpin
BrukerBiospin
collection
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1 詳細: SIMULATED ANNEALING WAS STARTED WITH 3500 AND COOL DOWN TO 100 K WITH 15,000 STEPS. STRUCTURE WAS ENERGY MINIMIZED WITH POWELL ENERGY MINIMIZATION.
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 20