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Open data
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Basic information
Entry | Database: PDB / ID: 2mx7 | ||||||
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Title | Solution structure of the internal EH domain of gamma-synergin | ||||||
![]() | Synergin gamma | ||||||
![]() | PROTEIN BINDING / EH domain / EF hand / NPF repeat | ||||||
Function / homology | ![]() clathrin coat of trans-Golgi network vesicle / AP-1 adaptor complex / intracellular protein transport / endocytosis / Golgi apparatus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Kovermann, M. / Weininger, U. / Loew, C. | ||||||
![]() | ![]() Title: Solution structure of the internal EH domain of gamma-synergin Authors: Kovermann, M. / Weininger, U. / Loew, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 343.2 KB | Display | ![]() |
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PDB format | ![]() | 283.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 406.3 KB | Display | ![]() |
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Full document | ![]() | 478.9 KB | Display | |
Data in XML | ![]() | 23 KB | Display | |
Data in CIF | ![]() | 36.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 12294.267 Da / Num. of mol.: 1 / Fragment: internal EH domain (UNP residues 279-388) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 1 mM [U-99% 13C; U-99% 15N] protein, 20 mM TRIS, 100 mM sodium chloride, 2 mM calcium chloride, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O | ||||||||||||||||||||
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Sample |
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Sample conditions | pH: 7.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR constraints | NOE constraints total: 1575 / Protein phi angle constraints total count: 93 / Protein psi angle constraints total count: 92 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 300 / Conformers submitted total number: 10 |