Mass: 8642.016 Da / Num. of mol.: 1 / Fragment: Residues 1-72 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Strain: K12 / Gene: hha, b0460, JW0449 / Production host: Escherichia coli (E. coli) / References: UniProt: P0ACE3
#2: Protein/peptide
DNA-bindingproteinH-NS / Histone-like protein HLP-II / Protein B1 / Protein H1
Mass: 5467.234 Da / Num. of mol.: 2 / Fragment: Residues 1-46 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Strain: K12 Gene: hns, bglY, cur, drdX, hnsA, msyA, osmZ, pilG, topS, b1237, JW1225 Production host: Escherichia coli (E. coli) / References: UniProt: P0ACF8
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
2
2D 1H-15N HSQC
2
3
3
2D 1H-15N HSQC
2
4
3
3D 1H-15N NOESY
2
5
3
3D 1H-15N TOCSY
2
6
4
2D 1H-15N HSQC
2
7
4
3D 1H-15N NOESY
2
8
4
3D 1H-15N TOCSY
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.015-0.030 mM protein_1, 0.10 mM [U-100% 15N] protein_2, 20 mM HEPES, 150 mM sodium chloride, 0.01 w/v sodium azide, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.015-0.030 mM protein_1, 0.10 mM [U-100% 15N] protein_2, 20 mM HEPES, 150 mM sodium chloride, 0.01 w/v sodium azide, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.9 mM [U-100% 15N] protein_1, 150 mM sodium chloride, 0.01 w/v sodium azide, 20 mM sodium phosphate, 90% H2O/10% D2O
90% H2O/10% D2O
4
0.9 mM [U-100% 15N] protein_2, 150 mM sodium chloride, 0.01 w/v sodium azide, 20 mM sodium phosphate, 90% H2O/10% D2O
90% H2O/10% D2O
Sample
Conc. (mg/ml)
Units
Component
Isotopic labeling
Conc. range (mg/ml)
Solution-ID
mM
entity_1-1
0.015-0.030
1
0.10mM
entity_2-2
[U-100% 15N]
1
20mM
HEPES-3
1
150mM
sodium chloride-4
1
0.01w/v
sodium azide-5
1
mM
entity_1-6
0.015-0.030
2
0.10mM
entity_2-7
[U-100% 15N]
2
20mM
HEPES-8
2
150mM
sodium chloride-9
2
0.01w/v
sodium azide-10
2
0.9mM
entity_1-11
[U-100% 15N]
3
150mM
sodium chloride-12
3
0.01w/v
sodium azide-13
3
20mM
sodium phosphate-14
3
0.9mM
entity_2-15
[U-100% 15N]
4
150mM
sodium chloride-16
4
0.01w/v
sodium azide-17
4
20mM
sodium phosphate-18
4
Sample conditions
Conditions-ID
Ionic strength
pH
Pressure (kPa)
Temperature (K)
1
0.16
7.0
ambient
295K
2
0.20
7.0
ambient
295K
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NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker Avance
Bruker
AVANCE
800
1
Bruker Avance III
Bruker
AVANCEIII
600
2
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Processing
NMR software
Name
Version
Developer
Classification
NMRPipe
Delaglio, ZhengrongandBax
processing
NMRView
Johnson, OneMoonScientific
peakpicking
CARA
KellerandWuthrich
chemicalshiftassignment
CNS
2
Brunger, Adams, Clore, Gros, NilgesandRead
calculationengine
HADDOCK
2.1
AlexandreBonvin
data-driven docking using cns as structure calculation engine
TopSpin
BrukerBiospin
nmrspectraacquisition
HADDOCK
2.1
AlexandreBonvin
refinement
CNS
2
Brunger, Adams, Clore, Gros, NilgesandRead
refinement
Refinement
Method: Rigid-body, torsion angle simulated annealing, Cartesian Molecular dynamics Software ordinal: 1 Details: HADDOCK modelling consists of (i) rigid-body docking, (ii) semi-flexible refinement stage and (iii) final refinement in explicit solvent., Final gentle water simulated annealing refinement ...Details: HADDOCK modelling consists of (i) rigid-body docking, (ii) semi-flexible refinement stage and (iii) final refinement in explicit solvent., Final gentle water simulated annealing refinement protocol using molecular dynamics in Cartesian space.
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: target function / Conformers calculated total number: 400 / Conformers submitted total number: 10
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