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- PDB-2mpo: Structural basis of Toxoplasma gondii MIC2-Associated Protein int... -

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Basic information

Entry
Database: PDB / ID: 2mpo
TitleStructural basis of Toxoplasma gondii MIC2-Associated Protein interaction with MIC2
ComponentsMIC2-associated protein
KeywordsCELL ADHESION / Toxoplasma gondii / Plasmodium / Microneme / Mic2 / M2AP
Function / homology: / MIC2-associated protein, central beta-domain / MIC2-associated protein
Function and homology information
Biological speciesToxoplasma gondii (eukaryote)
MethodSOLUTION NMR / simulated annealing, distance geometry
Model detailslowest energy, model1
AuthorsLiu, B. / Matthews, S.
CitationJournal: To Be Published
Title: Structural basis of Toxoplasma gondii MIC2-Associated Protein interaction with MIC2
Authors: Liu, B. / Huynh, M. / Henry, M. / Liew, L. / Matthews, S.J. / Carruthers, V.B.
History
DepositionMay 29, 2014Deposition site: BMRB / Processing site: PDBJ
Revision 1.0Nov 26, 2014Provider: repository / Type: Initial release
Revision 1.1Nov 20, 2024Group: Data collection / Database references / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: MIC2-associated protein


Theoretical massNumber of molelcules
Total (without water)19,3441
Polymers19,3441
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / 1structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein MIC2-associated protein


Mass: 19343.619 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 47-228
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Toxoplasma gondii (eukaryote) / Production host: Escherichia coli (E. coli) / References: UniProt: Q967S9
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D 1H-15N HSQC
1213D CBCA(CO)NH
1313D C(CO)NH
1413D HNCO
1513D HNCA
1613D HN(CA)CB
1713D (H)CCH-TOCSY
1813D H(CCO)NH
1913D 1H-13C NOESY
11013D 1H-15N NOESY

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Sample preparation

DetailsContents: 50 mM potassium phosphate-1, 100 mM sodium chloride-2, 90% H2O/10% D2O
Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)ComponentSolution-ID
50 mMpotassium phosphate-11
100 mMsodium chloride-21
Sample conditionsIonic strength: 0.1 / pH: 5.8 / Pressure: ambient / Temperature: 310 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker DRXBrukerDRX5001
Bruker DRXBrukerDRX8002

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Processing

NMR software
NameDeveloperClassification
ARIALinge, O'Donoghue and Nilgesstructure solution
ARIALinge, O'Donoghue and Nilgesrefinement
RefinementMethod: simulated annealing, distance geometry / Software ordinal: 1
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 1 / Conformers submitted total number: 1 / Representative conformer: 1

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