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Yorodumi- PDB-2mou: Solution structure of StAR-related lipid transfer domain protein ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mou | ||||||
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Title | Solution structure of StAR-related lipid transfer domain protein 6 (STARD6) | ||||||
Components | StAR-related lipid transfer protein 6 | ||||||
Keywords | TRANSPORT PROTEIN / Steroidogenic acute regulatory protein (StAR) / START domain / Cholesterol metabolism / Steroidogenesis | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | closest to the average, model1 | ||||||
Authors | Letourneau, D. / Bedard, M. / Lefebvre, A. / Lehoux, J.G. / Lavigne, P. | ||||||
Citation | Journal: To be Published Title: Solution structure of StAR-related lipid transfer domain protein 6 (STARD6) Authors: Letourneau, D. / Bedard, M. / Lefebvre, A. / Lehoux, J.G. / Lavigne, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mou.cif.gz | 1.5 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2mou.ent.gz | 1.3 MB | Display | PDB format |
PDBx/mmJSON format | 2mou.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mo/2mou ftp://data.pdbj.org/pub/pdb/validation_reports/mo/2mou | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 25057.555 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: NM_139171/NP_631910, STARD6 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P59095 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1 mM [U-100% 13C; U-100% 15N] StAR_D6, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1 mM / Component: StAR_D6-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 0.100 / pH: 7.000 / Pressure: 1.000 atm / Temperature: 298.150 K |
-NMR measurement
NMR spectrometer | Type: Varian UnityInova / Manufacturer: Varian / Model: UnityInova / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: Lowest energy, restraint violations and Ramachandran parameters Conformers calculated total number: 1000 / Conformers submitted total number: 20 |