THE SEQUENCE CONSTRUCTION WAS THE SAME AS DATABASE UNP D0KTR7_SULS9, BUT DERIVED FROM DIFFERENT ...THE SEQUENCE CONSTRUCTION WAS THE SAME AS DATABASE UNP D0KTR7_SULS9, BUT DERIVED FROM DIFFERENT STRAIN. C-TERMINAL LEHHHHHH ARE THE EXPRESSION TAGS.
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC
1
3
1
3D HNCO
1
4
1
3D HN(CA)CB
1
5
1
3DCBCA(CO)NH
1
6
1
3DHN(CA)CO
1
7
1
3DHBHA(CO)NH
1
8
1
3DHBHANH
1
9
1
3D CCH-TOCSY
1
10
1
3D 1H-15N TOCSY
1
11
1
3D 1H-15N NOESY
1
12
1
3D 1H-13C NOESY aliphatic
1
13
1
3D 1H-13C NOESY aromatic
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試料調製
詳細
内容: 0.5-1.0 mM [U-13C; U-15N] protein-1, 50 mM potassium phosphate-2, 50 mM potassium chloride-3, 2 mM EDTA-4, 0.02 % DSS-5, 90% H2O/10% D2O 溶媒系: 90% H2O/10% D2O
試料
濃度 (mg/ml)
単位
構成要素
Isotopic labeling
Conc. range (mg/ml)
Solution-ID
mM
entity-1
[U-13C; U-15N]
0.5-1.0
1
50mM
potassium phosphate-2
1
50mM
potassium chloride-3
1
2mM
EDTA-4
1
0.02 %
DSS-5
1
試料状態
イオン強度: 100 / pH: 6 / 圧: ambient / 温度: 298 K
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NMR測定
NMRスペクトロメーター
タイプ: Bruker DMX / 製造業者: Bruker / モデル: DMX / 磁場強度: 600 MHz