Mass: 6711.617 Da / Num. of mol.: 1 / Fragment: C-terminal domain (UNP residues 178-238) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Gene: nusG, Rv0639, MT0668, MTCY20H10.20 / Plasmid: pET101/D-TOPO / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P65589, UniProt: P9WIU9*PLUS
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC aliphatic
1
3
1
2D 1H-13C HSQC aromatic
1
4
1
3D HNCO
1
5
1
3D HNCA
1
6
1
3D HN(CA)CB
1
7
1
3DCBCA(CO)NH
1
8
1
3DC(CO)NH
1
9
1
3D (H)CCH-TOCSY
1
10
1
3D 1H-15N NOESY
1
11
1
3D 1H-13C NOESY
1
12
1
3D CCH-TOCSY
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Sample preparation
Details
Contents: 1.1 mM [U-95% 13C; U-90% 15N] protein, 25 mM HEPES, 150 mM sodium chloride, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
1.1mM
entity-1
[U-95% 13C; U-90% 15N]
1
25mM
HEPES-2
1
150mM
sodium chloride-3
1
Sample conditions
pH: 7.5 / Pressure: ambient / Temperature: 298 K
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NMR measurement
NMR spectrometer
Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz
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Processing
NMR software
Name
Developer
Classification
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
structuresolution
NMRView
Johnson, OneMoonScientific
nmrdataanalysis
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
refinement
Refinement
Method: simulated annealing / Software ordinal: 1
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 60 / Conformers submitted total number: 10 / Maximum upper distance constraint violation: 0.1 Å
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