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Yorodumi- PDB-2mgk: HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTAN... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mgk | ||||||
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Title | HIGH RESOLUTION CRYSTAL STRUCTURES OF FIVE DISTAL HISTIDINE MUTANTS OF SPERM WHALE MYOGLOBIN | ||||||
Components | MYOGLOBIN | ||||||
Keywords | OXYGEN STORAGE / carbon monoxide | ||||||
Function / homology | Function and homology information nitrite reductase activity / Oxidoreductases; Acting on other nitrogenous compounds as donors / sarcoplasm / Oxidoreductases; Acting on a peroxide as acceptor; Peroxidases / removal of superoxide radicals / oxygen carrier activity / oxygen binding / peroxidase activity / heme binding / extracellular exosome / metal ion binding Similarity search - Function | ||||||
Biological species | Physeter catodon (sperm whale) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Quillin, M.L. / Arduini, R.M. / Phillips Jr., G.N. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1993 Title: High-resolution crystal structures of distal histidine mutants of sperm whale myoglobin. Authors: Quillin, M.L. / Arduini, R.M. / Olson, J.S. / Phillips Jr., G.N. #1: Journal: Proteins / Year: 1990 Title: Crystal Structure of Myoglobin from a Synthetic Gene Authors: Phillips Jr., G.N. / Arduini, R.M. / Springer, B.A. / Sligar, S.G. #2: Journal: Proc.Natl.Acad.Sci.USA / Year: 1987 Title: High-Level Expression of Sperm Whale Myoglobin in Escherichia Coli Authors: Springer, B.A. / Sligar, S.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mgk.cif.gz | 47.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mgk.ent.gz | 33 KB | Display | PDB format |
PDBx/mmJSON format | 2mgk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2mgk_validation.pdf.gz | 834.1 KB | Display | wwPDB validaton report |
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Full document | 2mgk_full_validation.pdf.gz | 843.5 KB | Display | |
Data in XML | 2mgk_validation.xml.gz | 11.4 KB | Display | |
Data in CIF | 2mgk_validation.cif.gz | 15.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mg/2mgk ftp://data.pdbj.org/pub/pdb/validation_reports/mg/2mgk | HTTPS FTP |
-Related structure data
Related structure data | 1mobC 1mocC 1modC 2mgaC 2mgbC 2mgcC 2mgdC 2mgeC 2mgfC 2mggC 2mghC 2mgiC 2mgjC 2mglC 2mgmC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 17365.164 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Physeter catodon (sperm whale) / Gene: SYNTHETIC GENE / References: UniProt: P02185 |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-HEM / |
#4: Chemical | ChemComp-CMO / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.17 Å3/Da / Density % sol: 61.2 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 9 / Method: batch method / Details: micro seeding | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2 Å / % possible obs: 90 % / Rmerge(I) obs: 0.068 |
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-Processing
Software |
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Refinement | Resolution: 2→5 Å /
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Refinement step | Cycle: LAST / Resolution: 2→5 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.146 / Rfactor Rwork: 0.146 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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