[English] 日本語
Yorodumi- PDB-2m7g: Structure of the Type IVa Major Pilin from the Electrically Condu... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2m7g | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of the Type IVa Major Pilin from the Electrically Conductive Bacterial Nanowires of Geobacter sulfurreducens | ||||||
Components | Geopilin domain 1 protein | ||||||
Keywords | Cell Adhesion / Structural Protein / Electron Transport / pilin / PilA / bacterial nanowire / type IVa | ||||||
Function / homology | : Function and homology information | ||||||
Biological species | Geobacter sulfurreducens (bacteria) | ||||||
Method | SOLUTION NMR / torsion angle dynamics, simulated annealing | ||||||
Model details | fewest violations, model 10 | ||||||
Authors | Reardon, P.N. / Mueller, K.T. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2013 Title: Structure of the Type IVa Major Pilin from the Electrically Conductive Bacterial Nanowires of Geobacter sulfurreducens. Authors: Reardon, P.N. / Mueller, K.T. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2m7g.cif.gz | 367.6 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2m7g.ent.gz | 312 KB | Display | PDB format |
PDBx/mmJSON format | 2m7g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2m7g_validation.pdf.gz | 525 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 2m7g_full_validation.pdf.gz | 740.5 KB | Display | |
Data in XML | 2m7g_validation.xml.gz | 44.5 KB | Display | |
Data in CIF | 2m7g_validation.cif.gz | 46.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m7/2m7g ftp://data.pdbj.org/pub/pdb/validation_reports/m7/2m7g | HTTPS FTP |
-Related structure data
Similar structure data | |
---|---|
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 6576.502 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Geobacter sulfurreducens (bacteria) / Strain: DL-1 / KN400 / Gene: KN400_1523, pila, pilA-N / Production host: Escherichia coli (E. coli) / Strain (production host): C41(DE3) / References: UniProt: D7AIT1 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Contents: 1 mM [U-13C; U-15N] PilA, 200 mM [U-2H] DHPC, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||
Sample conditions | pH: 5 / Pressure: ambient / Temperature: 308 K |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| ||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: torsion angle dynamics, simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 755 / NOE intraresidue total count: 172 / NOE long range total count: 1 / NOE medium range total count: 329 / NOE sequential total count: 253 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 18 / Maximum upper distance constraint violation: 0.47 Å |