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Yorodumi- PDB-2m3k: Global fold of the type IV pilin ComP from Neisseria meningitidis -
+Open data
-Basic information
Entry | Database: PDB / ID: 2m3k | ||||||
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Title | Global fold of the type IV pilin ComP from Neisseria meningitidis | ||||||
Components | Minor pilin ComP | ||||||
Keywords | MOTOR PROTEIN / DNA uptake | ||||||
Function / homology | Glycoprotein, Type 4 Pilin - #50 / Glycoprotein, Type 4 Pilin / 2-Layer Sandwich / Alpha Beta / : Function and homology information | ||||||
Biological species | Neisseria meningitidis (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | closest to the average, model1 | ||||||
Authors | Simpson, P. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2013 Title: Specific DNA recognition mediated by a type IV pilin. Authors: Cehovin, A. / Simpson, P.J. / McDowell, M.A. / Brown, D.R. / Noschese, R. / Pallett, M. / Brady, J. / Baldwin, G.S. / Lea, S.M. / Matthews, S.J. / Pelicic, V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2m3k.cif.gz | 48.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2m3k.ent.gz | 38.1 KB | Display | PDB format |
PDBx/mmJSON format | 2m3k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2m3k_validation.pdf.gz | 499.7 KB | Display | wwPDB validaton report |
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Full document | 2m3k_full_validation.pdf.gz | 556.8 KB | Display | |
Data in XML | 2m3k_validation.xml.gz | 19.2 KB | Display | |
Data in CIF | 2m3k_validation.cif.gz | 14.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m3/2m3k ftp://data.pdbj.org/pub/pdb/validation_reports/m3/2m3k | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13512.774 Da / Num. of mol.: 1 / Fragment: UNP residues 35-149 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Neisseria meningitidis (bacteria) / Gene: comP, NMV_2216 / Production host: Escherichia coli (E. coli) / References: UniProt: C9X2N5 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: low resolution solution structure | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.1-0.5 mM [U-100% 13C; U-100% 15N] ComP, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Units: mM / Component: ComP-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] / Conc. range: 0.1-0.5 |
Sample conditions | Ionic strength: 0.05 / pH: 6.7 / Pressure: ambient / Temperature: 310 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: closest to the average | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 20 / Conformers submitted total number: 1 |