+Open data
-Basic information
Entry | Database: PDB / ID: 2m1n | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structure of a chaperone in type III secretion system | ||||||
Components | Type III secretion system filament chaperone CesA | ||||||
Keywords | CHAPERONE / Helix bundle | ||||||
Function / homology | : Function and homology information | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Chen, L. / Economou, A. / Kalodimos, C. | ||||||
Citation | Journal: Cell Rep / Year: 2013 Title: Substrate-Activated Conformational Switch on Chaperones Encodes a Targeting Signal in Type III Secretion. Authors: Chen, L. / Ai, X. / Portaliou, A.G. / Minetti, C.A. / Remeta, D.P. / Economou, A. / Kalodimos, C.G. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2m1n.cif.gz | 1.5 MB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2m1n.ent.gz | 1.3 MB | Display | PDB format |
PDBx/mmJSON format | 2m1n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2m1n_validation.pdf.gz | 559.9 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 2m1n_full_validation.pdf.gz | 911.9 KB | Display | |
Data in XML | 2m1n_validation.xml.gz | 91.5 KB | Display | |
Data in CIF | 2m1n_validation.cif.gz | 112.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m1/2m1n ftp://data.pdbj.org/pub/pdb/validation_reports/m1/2m1n | HTTPS FTP |
-Related structure data
Similar structure data | |
---|---|
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 12260.131 Da / Num. of mol.: 2 / Mutation: D11L, R15D, E17L Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: 3003 / Gene: EC3003_4066 / Production host: Escherichia coli (E. coli) / References: UniProt: I2YZH3 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Contents: 500 mM [U-13C; U-15N; U-2H], 13CH3-Ala, Ile, Val, Leu CesAB (Type III secretion system, chaperone for EspA and EspB), 93% H2O/7% D2O Solvent system: 93% H2O/7% D2O |
---|---|
Sample | Conc.: 500 mM Component: CesAB (Type III secretion system, chaperone for EspA and EspB)-1 Isotopic labeling: [U-13C; U-15N; U-2H], 13CH3-Ala, Ile, Val, Leu |
Sample conditions | Ionic strength: 0.3 / pH: 6.5 / Pressure: ambient / Temperature: 321 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 700 MHz |
---|
-Processing
NMR software |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |