+Open data
-Basic information
Entry | Database: PDB / ID: 2m1h | ||||||
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Title | Solution structure of a PWWP domain from Trypanosoma brucei | ||||||
Components | Transcription elongation factor S-II | ||||||
Keywords | TRANSCRIPTION / transcription factor | ||||||
Function / homology | Function and homology information DNA-templated transcription elongation / translation elongation factor activity / DNA-binding transcription factor activity / regulation of DNA-templated transcription / DNA binding / zinc ion binding / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | Trypanosoma brucei (eukaryote) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Wang, R. / Fan, K. / Liao, S. / Zhang, J. / Tu, X. | ||||||
Citation | Journal: Proteins / Year: 2016 Title: Solution structure of TbTFIIS2-1 PWWP domain from Trypanosoma brucei. Authors: Wang, R. / Zhang, J. / Liao, S. / Tu, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2m1h.cif.gz | 753 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2m1h.ent.gz | 643.7 KB | Display | PDB format |
PDBx/mmJSON format | 2m1h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m1/2m1h ftp://data.pdbj.org/pub/pdb/validation_reports/m1/2m1h | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13247.487 Da / Num. of mol.: 1 / Fragment: PWWP domain (UNP residues 1-110) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trypanosoma brucei (eukaryote) / Gene: Tb927.2.3580 / Plasmid: pET22 / Production host: Escherichia coli (E. coli) / References: UniProt: Q586X9 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.5 mM [U-100% 13C; U-100% 15N] tf21, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.5 mM / Component: tf21-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 0.15 / pH: 6.8 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 500 / Conformers submitted total number: 20 |