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Yorodumi- PDB-2lx0: Arced helix (ArcH) NMR structure of the reovirus p14 fusion-assoc... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lx0 | ||||||
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Title | Arced helix (ArcH) NMR structure of the reovirus p14 fusion-associated small transmembrane (FAST) protein transmembrane domain (TMD) in dodecyl phosphocholine (DPC) micelles | ||||||
Components | Membrane fusion protein p14Lipid bilayer fusion | ||||||
Keywords | MEMBRANE PROTEIN / Membrane fusion protein transmembrane domain / p14 FAST protein TMD / ArcH / micelle-peptide complex | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Sarker, M. / Key, T. / Duncan, R. / Rainey, J.K. | ||||||
Citation | Journal: To be Published Title: A Cell-Cell Membrane Fusion Module Comprising a Transmembrane Arced Helix (ArcH) Authors: Sarker, M. / Key, T. / Rainey, J.K. / Duncan, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lx0.cif.gz | 545.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lx0.ent.gz | 463.7 KB | Display | PDB format |
PDBx/mmJSON format | 2lx0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lx/2lx0 ftp://data.pdbj.org/pub/pdb/validation_reports/lx/2lx0 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3816.661 Da / Num. of mol.: 1 / Fragment: transmembrane domain / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.75 mM Partial 15N (8 of 32 amino acids) p14 TMD peptide, 150 mM [U-2H] DPC, 0.5 mM DSS, 0.2 mM sodium azide, 20 mM [U-2H] sodium acetate, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O | ||||||||||||||||||||||||
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Sample |
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Sample conditions | pH: 5.0 / Pressure: ambient / Temperature: 310 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 700 MHz |
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-Processing
NMR software |
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