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Yorodumi- PDB-2lnm: Solution structure of the C-terminal NP-repeat domain of Tic40, a... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lnm | ||||||
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Title | Solution structure of the C-terminal NP-repeat domain of Tic40, a co-chaperone during protein import into chloroplasts | ||||||
Components | Protein TIC 40, chloroplastic | ||||||
Keywords | PROTEIN TRANSPORT / translocon / import / chloroplast / Tic40-NP | ||||||
Function / homology | Function and homology information Tic complex / protein import into chloroplast stroma / chloroplast inner membrane / chloroplast organization / chloroplast envelope / plastid / chloroplast thylakoid membrane / chloroplast / membrane => GO:0016020 Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Chen, C. / Kao, Y. | ||||||
Citation | Journal: J.Biochem. / Year: 2012 Title: Solution structure of the C-terminal NP-repeat domain of Tic40, a co-chaperone during protein import into chloroplasts. Authors: Kao, Y.F. / Lou, Y.C. / Yeh, Y.H. / Hsiao, C.D. / Chen, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lnm.cif.gz | 376.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lnm.ent.gz | 314.7 KB | Display | PDB format |
PDBx/mmJSON format | 2lnm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ln/2lnm ftp://data.pdbj.org/pub/pdb/validation_reports/ln/2lnm | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7056.141 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 386-447 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Description: NdeI, XhoI / Gene: TIC40, PDE120, At5g16620, MTG13.6 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9FMD5 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 20 mM potassium phosphate-1, 20 mM sodium citrate-2, 100 mM sodium chloride-3, 10 % [U-99% 2H] TFE-4, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 140 / pH: 5.56 / Pressure: ambient / Temperature: 310 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 839 / NOE intraresidue total count: 158 / NOE long range total count: 85 / NOE medium range total count: 309 / NOE sequential total count: 257 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: lowest energy / Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 1 |