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- PDB-2ll2: Structure of the Cx43 C-terminal domain bound to tubulin -

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Basic information

Entry
Database: PDB / ID: 2ll2
TitleStructure of the Cx43 C-terminal domain bound to tubulin
ComponentsGap junction alpha-1 protein
KeywordsMEMBRANE PROTEIN
Function / homology
Function and homology information


gap junction channel activity involved in cardiac conduction electrical coupling / negative regulation of gonadotropin secretion / positive regulation of striated muscle tissue development / milk ejection reflex / positive regulation of morphogenesis of an epithelium / positive regulation of mesodermal cell differentiation / atrial ventricular junction remodeling / cell communication by chemical coupling / cell communication by electrical coupling / neuroblast migration ...gap junction channel activity involved in cardiac conduction electrical coupling / negative regulation of gonadotropin secretion / positive regulation of striated muscle tissue development / milk ejection reflex / positive regulation of morphogenesis of an epithelium / positive regulation of mesodermal cell differentiation / atrial ventricular junction remodeling / cell communication by chemical coupling / cell communication by electrical coupling / neuroblast migration / columnar/cuboidal epithelial cell maturation / gap junction hemi-channel activity / negative regulation of trophoblast cell migration / regulation of bone remodeling / microtubule-based transport / monoatomic ion transmembrane transporter activity / SARS-CoV-2 targets PDZ proteins in cell-cell junction / regulation of ventricular cardiac muscle cell membrane depolarization / gap junction channel activity involved in cell communication by electrical coupling / contractile muscle fiber / Oligomerization of connexins into connexons / Transport of connexins along the secretory pathway / glutathione transmembrane transporter activity / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / gap junction assembly / cellular response to pH / atrial cardiac muscle cell action potential / Regulation of gap junction activity / skeletal muscle tissue regeneration / cardiac conduction system development / connexin complex / regulation of atrial cardiac muscle cell membrane depolarization / Formation of annular gap junctions / Gap junction degradation / Golgi-associated vesicle membrane / fascia adherens / cell-cell contact zone / gap junction / Gap junction assembly / gap junction channel activity / bone remodeling / export across plasma membrane / adult heart development / xenobiotic transport / regulation of bone mineralization / glutamate secretion / regulation of ventricular cardiac muscle cell membrane repolarization / tight junction / blood vessel morphogenesis / lens development in camera-type eye / cell communication by electrical coupling involved in cardiac conduction / intermediate filament / embryonic digit morphogenesis / maintenance of blood-brain barrier / beta-tubulin binding / RHOJ GTPase cycle / positive regulation of stem cell proliferation / heart looping / RHOQ GTPase cycle / efflux transmembrane transporter activity / establishment of mitotic spindle orientation / intercalated disc / lateral plasma membrane / alpha-tubulin binding / T cell proliferation / monoatomic ion transmembrane transport / positive regulation of vascular associated smooth muscle cell proliferation / tubulin binding / protein tyrosine kinase binding / neuron migration / bone development / protein localization / negative regulation of cell growth / beta-catenin binding / osteoblast differentiation / cellular response to amyloid-beta / male gonad development / cell junction / cell-cell signaling / positive regulation of cold-induced thermogenesis / heart development / scaffold protein binding / spermatogenesis / positive regulation of canonical NF-kappaB signal transduction / in utero embryonic development / membrane raft / apical plasma membrane / Golgi membrane / negative regulation of gene expression / intracellular membrane-bounded organelle / focal adhesion / signaling receptor binding / positive regulation of gene expression / endoplasmic reticulum membrane / Golgi apparatus / signal transduction / mitochondrion / nucleoplasm / nucleus / plasma membrane
Similarity search - Function
Gap junction alpha-1 protein (Cx43) / Gap junction alpha-1 protein (Cx43), C-terminal / Gap junction alpha-1 protein (Cx43), alpha helix domain superfamily / Gap junction alpha-1 protein (Cx43) / Connexin, C-terminal / Connexin / Connexin, N-terminal / Connexin, conserved site / Gap junction protein, cysteine-rich domain / Connexin, N-terminal domain superfamily ...Gap junction alpha-1 protein (Cx43) / Gap junction alpha-1 protein (Cx43), C-terminal / Gap junction alpha-1 protein (Cx43), alpha helix domain superfamily / Gap junction alpha-1 protein (Cx43) / Connexin, C-terminal / Connexin / Connexin, N-terminal / Connexin, conserved site / Gap junction protein, cysteine-rich domain / Connexin, N-terminal domain superfamily / Connexin / Connexins signature 1. / Connexins signature 2. / Connexin homologues / Gap junction channel protein cysteine-rich domain
Similarity search - Domain/homology
Gap junction alpha-1 protein
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR / simulated annealing
Model detailslowest energy, model 10
AuthorsSaidi Brikci-Nigassa, A. / Clement, M. / Ha-Duong, T. / Benmansour, K. / Adjadj, E. / Ziani, L. / Pastre, D. / Curmi, P.A.
CitationJournal: Biochemistry / Year: 2012
Title: Phosphorylation controls the interaction of the connexin43 C-terminal domain with tubulin and microtubules.
Authors: Saidi Brikci-Nigassa, A. / Clement, M.J. / Ha-Duong, T. / Adjadj, E. / Ziani, L. / Pastre, D. / Curmi, P.A. / Savarin, P.
History
DepositionOct 26, 2011Deposition site: BMRB / Processing site: RCSB
Revision 1.0Jun 20, 2012Provider: repository / Type: Initial release
Revision 1.1Jun 14, 2023Group: Data collection / Database references / Other
Category: database_2 / pdbx_database_status / pdbx_nmr_spectrometer
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_nmr_data / _pdbx_nmr_spectrometer.model
Revision 1.2May 15, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2 / Item: _database_2.pdbx_DOI

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Gap junction alpha-1 protein


Theoretical massNumber of molelcules
Total (without water)2,7201
Polymers2,7201
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)10 / 100structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein/peptide Gap junction alpha-1 protein / Connexin-43 / Cx43 / Gap junction 43 kDa heart protein


Mass: 2720.067 Da / Num. of mol.: 1 / Fragment: Microtubule binding domain residues 234-259 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P17302

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR / Details: Structure of the 234-259 of Cx43 bound to tubulin
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D 1H-1H TOCSY
1212D 1H-1H NOESY
1322D 1H-1H TOCSY
1422D 1H-1H NOESY
1521D STD
1611D STD

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Sample preparation

Details
Solution-IDContentsSolvent system
10.8 mM protein, 0.04 mM [U-100% 15N] D2O, 50 mM [U-100% 15N] PIPES, 95% H2O/5% D2O95% H2O/5% D2O
20.8 mM protein, 0.04 mM [U-100% 15N] D2O, 50 mM [U-100% 15N] PIPES, 0.04 mM tubulin, 95% H2O/5% D2O95% H2O/5% D2O
Sample
Conc. (mg/ml)ComponentIsotopic labelingSolution-ID
0.8 mMentity-11
0.04 mMD2O-2[U-100% 15N]1
50 mMPIPES-3[U-100% 15N]1
0.8 mMentity-42
0.04 mMD2O-5[U-100% 15N]2
50 mMPIPES-6[U-100% 15N]2
0.04 mMtubulin-72
Sample conditionsIonic strength: 0 / pH: 6.9 / Pressure: ambient / Temperature: 282 K

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NMR measurement

NMR spectrometerType: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz

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Processing

NMR software
NameVersionDeveloperClassification
NMRView5.2Johnson, One Moon Scientificchemical shift assignment
NMRView5.2Johnson, One Moon Scientificpeak picking
CNSBrunger, Adams, Clore, Gros, Nilges and Readrefinement
RefinementMethod: simulated annealing / Software ordinal: 1
NMR constraintsNOE constraints total: 425 / NOE intraresidue total count: 199 / NOE long range total count: 5 / NOE medium range total count: 69 / NOE sequential total count: 152
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 100 / Conformers submitted total number: 10 / Maximum upper distance constraint violation: 0.56 Å

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