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Yorodumi- PDB-2lj3: PFBD: High-throughput Strategy of Backbone fold Determination for... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lj3 | ||||||
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Title | PFBD: High-throughput Strategy of Backbone fold Determination for small well-folded proteins in less than a day | ||||||
Components | Spectrin alpha chain, brain | ||||||
Keywords | STRUCTURAL PROTEIN / Chicken SH3 Domain | ||||||
Function / homology | Function and homology information costamere / actin filament capping / cortical actin cytoskeleton / cell projection / actin filament binding / cell junction / actin cytoskeleton organization / calmodulin binding / calcium ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Kumar, D. / Hosur, R. | ||||||
Citation | Journal: To be Published Title: PFBD: High-throughput Strategy of Backbone fold Determination for small well-folded proteins in less than a day Authors: Kumar, D. / Hosur, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lj3.cif.gz | 458.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lj3.ent.gz | 393 KB | Display | PDB format |
PDBx/mmJSON format | 2lj3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lj/2lj3 ftp://data.pdbj.org/pub/pdb/validation_reports/lj/2lj3 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7227.252 Da / Num. of mol.: 1 / Fragment: SH3 Domain (UNP RESIDUES 965-1025) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / Gene: SPTAN1, SPTA2 / Production host: Escherichia coli (E. coli) / References: UniProt: P07751 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: NMR Method Developments | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.00 mM [U-99% 13C; U-99% 15N] sodium phosphate-1, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1.00 mM / Component: sodium phosphate-1 / Isotopic labeling: [U-99% 13C; U-99% 15N] |
Sample conditions | Ionic strength: 50 / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 200 / Conformers submitted total number: 20 / Representative conformer: 1 |