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Yorodumi- PDB-2lhj: NMR structure of the high mobility group protein-like protein NHP... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lhj | ||||||
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Title | NMR structure of the high mobility group protein-like protein NHP1 from Babesia bovis T2Bo (BaboA.00841.a) | ||||||
Components | High mobility group protein homolog NHP1High-mobility group | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / Seattle Structural Genomics Center for Infectious Disease / SSGCID | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Babesia bovis (eukaryote) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Barnwal, R. / Varani, G. / Seattle Structural Genomics Center for Infectious Disease (SSGCID) | ||||||
Citation | Journal: To be Published Title: 1H and 15N Assigned Chemical Shifts for high mobility group protein-like protein NHP1 from Babesia bovis Authors: Barnwal, R. / Varani, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lhj.cif.gz | 350.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lhj.ent.gz | 300.1 KB | Display | PDB format |
PDBx/mmJSON format | 2lhj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lh/2lhj ftp://data.pdbj.org/pub/pdb/validation_reports/lh/2lhj | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11140.752 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Babesia bovis (eukaryote) / Production host: Escherichia coli (E. coli) / References: UniProt: P40632 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.2 mM [U-98% 13C; U-98% 15N] high mobility group protein-like protein NHP1, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1.2 mM / Component: high mobility group protein-like protein NHP1-1 / Isotopic labeling: [U-98% 13C; U-98% 15N] |
Sample conditions | pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 200 / Conformers submitted total number: 10 |