H/ACA ribonucleoprotein complex, subunit Nhp2-like / Ribosomal protein L30/S12 / 60s Ribosomal Protein L30; Chain: A; / Ribosomal protein L7Ae conserved site / Ribosomal protein L7Ae signature. / Ribosomal protein L7Ae/L8/Nhp2 family / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / 50S ribosomal protein L30e-like / 2-Layer Sandwich / Alpha Beta 類似検索 - ドメイン・相同性
20 mM [U-13C; U-15N] HEPES, 200 mM [U-13C; U-15N] potassium chloride, 1 mM [U-13C; U-15N] DTT, 100% D2O
100% D2O
2
20 mM [U-15N] HEPES, 200 mM [U-15N] potassium chloride, 1 mM [U-15N] DTT, 90% H2O/10% D2O
90% H2O/10% D2O
3
20 mM [U-13C; U-15N] HEPES, 200 mM [U-13C; U-15N] potassium chloride, 1 mM [U-13C; U-15N] DTT, 100% D2O
100% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
20mM
HEPES-1
[U-13C; U-15N]
1
200mM
potassium chloride-2
[U-13C; U-15N]
1
1mM
DTT-3
[U-13C; U-15N]
1
20mM
HEPES-4
[U-15N]
2
200mM
potassium chloride-5
[U-15N]
2
1mM
DTT-6
[U-15N]
2
20mM
HEPES-7
[U-13C; U-15N]
3
200mM
potassium chloride-8
[U-13C; U-15N]
3
1mM
DTT-9
[U-13C; U-15N]
3
試料状態
イオン強度: 0.2 / pH: 7.4 / 圧: ambient / 温度: 298 K
-
NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker DRX
Bruker
DRX
800
1
Bruker DRX
Bruker
DRX
600
2
-
解析
NMR software
名称
バージョン
開発者
分類
CYANA
2.1
Guntert, MumenthalerandWuthrich
構造決定
Sparky
Goddard
chemicalshiftassignment
Sparky
Goddard
データ解析
Sparky
Goddard
peakpicking
TopSpin
BrukerBiospin
collection
ProcheckNMR
LaskowskiandMacArthur
データ解析
TALOS
Cornilescu, DelaglioandBax
geometryoptimization
XwinNMR
BrukerBiospin
collection
CYANA
2.1
Guntert, MumenthalerandWuthrich
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1
NMR constraints
NOE constraints total: 915 / NOE long range total count: 208 / NOE medium range total count: 140 / NOE sequential total count: 567 / Protein phi angle constraints total count: 89 / Protein psi angle constraints total count: 88
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20
NMR ensemble rms
Distance rms dev: 0.74 Å / Distance rms dev error: 0.16 Å