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Yorodumi- PDB-2lbd: LIGAND-BINDING DOMAIN OF THE HUMAN RETINOIC ACID RECEPTOR GAMMA B... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2lbd | ||||||
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Title | LIGAND-BINDING DOMAIN OF THE HUMAN RETINOIC ACID RECEPTOR GAMMA BOUND TO ALL-TRANS RETINOIC ACID | ||||||
Components | RETINOIC ACID RECEPTOR GAMMA | ||||||
Keywords | NUCLEAR RECEPTOR / RETINOIC ACID RECEPTOR / ALL-TRANS RETINOIC ACID / LIGAND-BINDING DOMAIN / COMPLEX / HOLO FORM / TRANSCRIPTION REGULATION / LIGAND-DEPENDENT / ACTIVE CONFORMATION / Structural Proteomics in Europe / SPINE / Structural Genomics | ||||||
Function / homology | Function and homology information Harderian gland development / regulation of myeloid cell differentiation / trachea cartilage development / growth plate cartilage chondrocyte growth / embryonic eye morphogenesis / embryonic camera-type eye development / glandular epithelial cell development / prostate gland epithelium morphogenesis / negative regulation of chondrocyte differentiation / embryonic hindlimb morphogenesis ...Harderian gland development / regulation of myeloid cell differentiation / trachea cartilage development / growth plate cartilage chondrocyte growth / embryonic eye morphogenesis / embryonic camera-type eye development / glandular epithelial cell development / prostate gland epithelium morphogenesis / negative regulation of chondrocyte differentiation / embryonic hindlimb morphogenesis / positive regulation of programmed cell death / anterior/posterior pattern specification / regulation of myelination / Signaling by Retinoic Acid / regulation of cell size / face development / canonical Wnt signaling pathway / retinoic acid receptor signaling pathway / nuclear retinoid X receptor binding / response to retinoic acid / negative regulation of stem cell proliferation / cellular response to retinoic acid / cellular response to leukemia inhibitory factor / hormone-mediated signaling pathway / stem cell proliferation / neural tube closure / multicellular organism growth / Activation of anterior HOX genes in hindbrain development during early embryogenesis / Nuclear Receptor transcription pathway / nuclear receptor activity / sequence-specific double-stranded DNA binding / transcription regulator complex / cell differentiation / DNA-binding transcription factor activity, RNA polymerase II-specific / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / apoptotic process / chromatin binding / positive regulation of cell population proliferation / chromatin / positive regulation of gene expression / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleoplasm / membrane / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SIR / Resolution: 2.06 Å | ||||||
Authors | Renaud, J.-P. / Rochel, N. / Ruff, M. / Moras, D. / Structural Proteomics in Europe (SPINE) | ||||||
Citation | Journal: Nature / Year: 1995 Title: Crystal structure of the RAR-gamma ligand-binding domain bound to all-trans retinoic acid. Authors: Renaud, J.P. / Rochel, N. / Ruff, M. / Vivat, V. / Chambon, P. / Gronemeyer, H. / Moras, D. #1: Journal: Nature / Year: 1995 Title: Crystal Structure of the Ligand-Binding Domain of the Human Nuclear Receptor Rxr-Alpha Authors: Bourguet, W. / Ruff, M. / Chambon, P. / Gronemeyer, H. / Moras, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2lbd.cif.gz | 77.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2lbd.ent.gz | 58.2 KB | Display | PDB format |
PDBx/mmJSON format | 2lbd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lb/2lbd ftp://data.pdbj.org/pub/pdb/validation_reports/lb/2lbd | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 30193.055 Da / Num. of mol.: 1 / Fragment: LBD (LIGAND-BINDING DOMAIN), RESIDUES 178 - 423 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) Description: CDNA CLONING\: KRUST ET AL., PROC.NATL.ACAD.SCI.USA,86,5310-5314,1989 Cell line: BL21 / Cellular location: NUCLEUSCell nucleus / Gene: HUMAN RAR GAMMA A CDNA (NUCLEOTIDES 946 - 1683) / Plasmid: PET-15B / Species (production host): Escherichia coli / Cellular location (production host): CYTOPLASM / Gene (production host): HRARGAMMA / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P22932, UniProt: P13631*PLUS |
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#2: Chemical | ChemComp-REA / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 3 |
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-Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 33 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 7 Details: VAPOR DIFFUSION METHOD, HANGING DROP TECHNIQUE 5UL PROTEIN SOLUTION + 5UL RESERVOIR AGAINST 500UL RESERVOIR, pH 7.0, vapor diffusion - hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 15 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 268 K |
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Diffraction source | Source: SYNCHROTRON / Site: LURE / Beamline: DW32 / Wavelength: 0.901 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 1, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.901 Å / Relative weight: 1 |
Reflection | Resolution: 2.06→7.99 Å / Num. obs: 17193 / % possible obs: 94.08 % / Observed criterion σ(I): 0 / Redundancy: 3.77 % / Rmerge(I) obs: 0.0974 / Net I/σ(I): 22.8 |
Reflection shell | Resolution: 2.06→2.1 Å / Redundancy: 1.9 % / Rmerge(I) obs: 0.3323 / Mean I/σ(I) obs: 3.64 / % possible all: 57.5 |
-Processing
Software |
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Refinement | Method to determine structure: SIR / Resolution: 2.06→8 Å / Cross valid method: THROUGHOUT / σ(F): 3
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Displacement parameters | Biso mean: 21.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.06→8 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.21 / Rfactor Rwork: 0.21 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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