手法: 溶液NMR 詳細: Spatial structure of dimeric ErbB3 transmembrane domain
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
3D HNCO
1
2
1
3D HNCA
1
3
1
3DHN(CO)CA
1
4
1
3D 1H-15N NOESY
1
5
2
3D 1H-13C NOESY
1
6
1
2D 1H-15N HSQC
1
7
2
2D 1H-13C HSQC aliphatic
1
8
2
2D 1H-13C HSQC aromatic
1
9
2
3D 15N,13C-filtered, 13C-edited NOESY
1
10
1
3D 15N,13C-filtered, 13C-edited NOESY
-
試料調製
詳細
Solution-ID
内容
溶媒系
1
1 mM [U-100% 13C; U-100% 15N] ErbB3tm-1, 1 mM ErbB3tm-2, 80 mM [U-99% 2H] DPC-3, 1 mM sodium azide-4, 90% H2O/10% D2O
90% H2O/10% D2O
2
1 mM [U-100% 13C; U-100% 15N] ErbB3tm-5, 1 mM ErbB3tm-6, 80 mM [U-99% 2H] DPC-7, 1 mM sodium azide-8, 100% D2O
100% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
1mM
ErbB3tm-1
[U-100% 13C; U-100% 15N]
1
1mM
ErbB3tm-2
1
80mM
DPC-3
[U-99% 2H]
1
1mM
sodium azide-4
1
1mM
ErbB3tm-5
[U-100% 13C; U-100% 15N]
2
1mM
ErbB3tm-6
2
80mM
DPC-7
[U-99% 2H]
2
1mM
sodium azide-8
2
試料状態
イオン強度: 50 / pH: 5.5 / 圧: ambient / 温度: 313 K
-
NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker Avance
Bruker
AVANCE
800
1
Bruker Avance
Bruker
AVANCE
600
2
-
解析
NMR software
名称
バージョン
開発者
分類
CYANA
3
Guntert, MumenthalerandWuthrich
精密化
CARA
1.8.5
KellerandWuthrich
chemicalshiftassignment
CARA
1.8.5
KellerandWuthrich
データ解析
CARA
1.8.5
KellerandWuthrich
peakpicking
TopSpin
2.1
BrukerBiospin
解析
精密化
手法: simulated annealing / ソフトェア番号: 1
NMR constraints
NOE constraints total: 892 / NOE intraresidue total count: 444 / NOE long range total count: 30 / NOE medium range total count: 182 / NOE sequential total count: 236
代表構造
選択基準: fewest violations
NMRアンサンブル
Average torsion angle constraint violation: 0.6 ° / コンフォーマー選択の基準: target function / 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20 / Maximum lower distance constraint violation: 0 Å / Maximum torsion angle constraint violation: 6 ° / Maximum upper distance constraint violation: 0.38 Å / 代表コンフォーマー: 1