- PDB-2l69: Solution NMR Structure of de novo designed protein, P-loop NTPase... -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2l69
Title
Solution NMR Structure of de novo designed protein, P-loop NTPase fold, Northeast Structural Genomics Consortium Target OR28
Components
Rossmann 2x3 fold protein
Keywords
DE NOVO PROTEIN / Structural Genomics / NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) / PSI-Biology / Protein Structure Initiative / Unknown function
Mass: 15857.213 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) artificial gene (others) / Production host: Escherichia coli (E. coli)
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
2
2D 1H-13C HSQC
1
3
1
3D HNCO
1
4
1
3DCBCA(CO)NH
1
5
1
3D HN(CA)CB
1
6
1
3D 1H-13C arom NOESY
1
7
1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.62 mM [U-100% 13C; U-100% 15N] OR28, 95% H2O/5% D2O
95% H2O/5% D2O
2
0.47 mM [U-5% 13C; U-100% 15N] OR28, 95% H2O/5% D2O