- PDB-2l2d: Solution NMR Structure of human UBA-like domain of OTUD7A_11_83, ... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2l2d
Title
Solution NMR Structure of human UBA-like domain of OTUD7A_11_83, NESG target HT6304A/OCSP target OTUD7A_11_83/SGC-Toronto
Components
OTU domain-containing protein 7A
Keywords
HYDROLASE / uba fold / Structural Genomics / PSI-Biology / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG / Ontario Centre for Structural Proteomics / OCSP / SGC
Function / homology
Function and homology information
cysteine-type deubiquitinase activity => GO:0004843 / protein deubiquitination involved in ubiquitin-dependent protein catabolic process / protein K11-linked deubiquitination / protein K48-linked deubiquitination / protein K63-linked deubiquitination / protein deubiquitination / K63-linked polyubiquitin modification-dependent protein binding / negative regulation of canonical NF-kappaB signal transduction / Ovarian tumor domain proteases / ubiquitinyl hydrolase 1 ...cysteine-type deubiquitinase activity => GO:0004843 / protein deubiquitination involved in ubiquitin-dependent protein catabolic process / protein K11-linked deubiquitination / protein K48-linked deubiquitination / protein K63-linked deubiquitination / protein deubiquitination / K63-linked polyubiquitin modification-dependent protein binding / negative regulation of canonical NF-kappaB signal transduction / Ovarian tumor domain proteases / ubiquitinyl hydrolase 1 / cysteine-type deubiquitinase activity / DNA binding / zinc ion binding / nucleus / cytosol / cytoplasm Similarity search - Function
OTUdomain-containingprotein7A / Zinc finger protein Cezanne 2
Mass: 8045.925 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: OTUD7A, C15orf16, CEZANNE2, OTUD7 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8TE49, ubiquitinyl hydrolase 1
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
3D HNCO
1
2
1
3DCBCA(CO)NH
1
3
1
3DHBHA(CO)NH
1
4
1
3D HNCA
1
5
1
3D (H)CCH-TOCSY
1
6
1
3D CCH-TOCSY
1
7
1
3D 1H-15N NOESY
1
8
1
3D 1H-13C NOESY
1
9
1
3D 1H-13C NOESY aromatic
1
10
2
2D 1H-13C HSQC
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.5 mM [U-100% 13C; U-100% 15N] OTUD7A_11_83, 10 mM [U-100% 2H] TRIS, 300 mM sodium chloride, 10 uM zinc sulphate, 10 mM [U-100% 2H] DTT, 10 mM benzamidine, 1 x inhibitor cocktail, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.5 mM [U-10% 13C; U-99% 15N] OTUD7A_11_83, 10 mM [U-100% 2H] TRIS, 300 mM sodium chloride, 10 uM zinc sulphate, 10 mM [U-100% 2H] DTT, 10 mM benzamidine, 1 x inhibitor cocktail, 90% H2O/10% D2O
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