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Yorodumi- PDB-2kz7: Solution structure of the CARMIL CAH3a/b domain bound to capping ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kz7 | ||||||
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Title | Solution structure of the CARMIL CAH3a/b domain bound to capping protein (CP) | ||||||
Components |
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Keywords | PROTEIN BINDING / TROSY / Paramagnetic relaxation enhancement / protein-protein complex | ||||||
Function / homology | Function and homology information barbed-end actin filament uncapping / positive regulation of lamellipodium organization / negative regulation of barbed-end actin filament capping / Advanced glycosylation endproduct receptor signaling / RHOD GTPase cycle / RHOF GTPase cycle / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / COPI-independent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / macropinosome ...barbed-end actin filament uncapping / positive regulation of lamellipodium organization / negative regulation of barbed-end actin filament capping / Advanced glycosylation endproduct receptor signaling / RHOD GTPase cycle / RHOF GTPase cycle / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / COPI-independent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / macropinosome / Factors involved in megakaryocyte development and platelet production / actin filament network formation / WASH complex / sperm connecting piece / F-actin capping protein complex / macropinocytosis / negative regulation of filopodium assembly / urate metabolic process / regulation of Arp2/3 complex-mediated actin nucleation / Factors involved in megakaryocyte development and platelet production / cell junction assembly / ruffle organization / barbed-end actin filament capping / actin polymerization or depolymerization / regulation of cell morphogenesis / regulation of lamellipodium assembly / intermediate filament cytoskeleton / lamellipodium assembly / establishment or maintenance of cell polarity / cortical cytoskeleton / positive regulation of actin filament polymerization / cell leading edge / filamentous actin / brush border / positive regulation of substrate adhesion-dependent cell spreading / cytoskeleton organization / positive regulation of stress fiber assembly / hippocampal mossy fiber to CA3 synapse / Schaffer collateral - CA1 synapse / cell morphogenesis / Z disc / actin filament binding / cell migration / lamellipodium / actin cytoskeleton organization / postsynaptic density / positive regulation of cell migration / nuclear speck / protein-containing complex binding / nucleoplasm / membrane / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / molecular dynamics, simulated annealing, torsion angle dynamics | ||||||
Authors | Zwolak, A. / Tjandra, N. | ||||||
Citation | Journal: To be Published Title: Solution structure of the CARMIL CAH3a/b domain bound to capping protein (CP) Authors: Zwolak, A. / Uruno, T. / Piszczek, G.P. / Hammer III, J.A. / Tjandra, N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kz7.cif.gz | 1.9 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2kz7.ent.gz | 1.7 MB | Display | PDB format |
PDBx/mmJSON format | 2kz7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kz/2kz7 ftp://data.pdbj.org/pub/pdb/validation_reports/kz/2kz7 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 33001.789 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / Gene: CAPZA1 / Plasmid: PET / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P13127 |
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#2: Protein | Mass: 31403.449 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / Gene: CAPZB / Plasmid: pET / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P14315 |
#3: Protein | Mass: 9449.754 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Lrrc16a, Carmil, Lrrc16 / Plasmid: pGEX4 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: Q6EDY6 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 100 / pH: 6.5 / Pressure: ambient / Temperature: 305 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: molecular dynamics, simulated annealing, torsion angle dynamics Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 10 |