1.0 mM [U-100% 13C; U-100% 15N] PtR41O-1, 100 mM sodium chloride-2, 5 mM calcium chloride-3, 10 mM DTT-4, 20 mM MES-5, 0.02 % NaN3-6, 50 uM DSS-7, 90% H2O/10% D2O
90% H2O/10% D2O
2
1.2 mM [U-5% 13C; U-100% 15N] PtR41O-8, 100 mM sodium chloride-9, 5 mM calcium chloride-10, 10 mM DTT-11, 20 mM MES-12, 0.02 % NaN3-13, 50 uM DSS-14, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.66 mM [U-5% 13C; U-100% 15N] PtR41O-15, 66 mM sodium chloride-16, 3.3 mM calcium chloride-17, 6.6 mM DTT-18, 13.2 mM MES-19, 0.0132 % NaN3-20, 33 uM DSS-21, 4 % poly ethylene glycol-22, 83% H2O/17% D2O
83% H2O/17% D2O
4
0.66 mM [U-5% 13C; U-100% 15N] PtR41O-23, 66 mM sodium chloride-24, 3.3 mM calcium chloride-25, 6.6 mM DTT-26, 13.2 mM MES-27, 0.0132 % NaN3-28, 33 uM DSS-29, 13.25 mg/mL Pf1 phage-30, 83% H2O/17% D2O