Mass: 9308.923 Da / Num. of mol.: 1 / Fragment: sequence database residues 1287-1365 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pediococcus pentosaceus (bacteria) / Strain: ATCC 25745 / Gene: PEPE_0118 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)pMgK / References: UniProt: Q03HU7
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C CT-HSQC ali
1
3
1
2D 1H-13C CT-HSQC aro
1
4
1
3D HNCO
1
5
1
3DHN(CA)CO
1
6
1
GFTCBCA(CO)NH
1
7
1
GFT HN(CA)CB
1
8
1
3DHBHA(CO)NH
1
9
1
3D (H)CCH-TOCSY
1
10
1
3D (H)CCH-COSY ali
1
11
1
13C/15N-NOESY
1
12
2
2D 1H-13C CT-HSQC (methyl)
1
13
2
2D J-modulation 1H-15N HSQC
1
14
3
2D J-modulation 1H-15N HSQC
1
15
4
2D J-modulation 1H-15N HSQC
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
1.0 mM [U-100% 13C; U-100% 15N] PtR41O-1, 100 mM sodium chloride-2, 5 mM calcium chloride-3, 10 mM DTT-4, 20 mM MES-5, 0.02 % NaN3-6, 50 uM DSS-7, 90% H2O/10% D2O
90% H2O/10% D2O
2
1.2 mM [U-5% 13C; U-100% 15N] PtR41O-8, 100 mM sodium chloride-9, 5 mM calcium chloride-10, 10 mM DTT-11, 20 mM MES-12, 0.02 % NaN3-13, 50 uM DSS-14, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.66 mM [U-5% 13C; U-100% 15N] PtR41O-15, 66 mM sodium chloride-16, 3.3 mM calcium chloride-17, 6.6 mM DTT-18, 13.2 mM MES-19, 0.0132 % NaN3-20, 33 uM DSS-21, 4 % poly ethylene glycol-22, 83% H2O/17% D2O
83% H2O/17% D2O
4
0.66 mM [U-5% 13C; U-100% 15N] PtR41O-23, 66 mM sodium chloride-24, 3.3 mM calcium chloride-25, 6.6 mM DTT-26, 13.2 mM MES-27, 0.0132 % NaN3-28, 33 uM DSS-29, 13.25 mg/mL Pf1 phage-30, 83% H2O/17% D2O