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Yorodumi- PDB-2kwd: Supramolecular Protein Structure Determination by Site-Specific L... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kwd | ||||||
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Title | Supramolecular Protein Structure Determination by Site-Specific Long-Range Intermolecular Solid State NMR Spectroscopy | ||||||
Components | Immunoglobulin G-binding protein G | ||||||
Keywords | IMMUNE SYSTEM / GB1 / Crystal Packing / Solid-State / Quaternary Structure / TEDOR | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Streptococcus sp. 'group G' (bacteria) | ||||||
Method | SOLID-STATE NMR / simulated annealing | ||||||
Model details | lowest energy, model 2 | ||||||
Authors | Nieuwkoop, A.J. / Rienstra, C.M. | ||||||
Citation | Journal: J.Am.Chem.Soc. / Year: 2010 Title: Supramolecular protein structure determination by site-specific long-range intermolecular solid state NMR spectroscopy. Authors: Nieuwkoop, A.J. / Rienstra, C.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kwd.cif.gz | 833.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kwd.ent.gz | 710.3 KB | Display | PDB format |
PDBx/mmJSON format | 2kwd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2kwd_validation.pdf.gz | 405.8 KB | Display | wwPDB validaton report |
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Full document | 2kwd_full_validation.pdf.gz | 688.6 KB | Display | |
Data in XML | 2kwd_validation.xml.gz | 63.1 KB | Display | |
Data in CIF | 2kwd_validation.cif.gz | 91 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kw/2kwd ftp://data.pdbj.org/pub/pdb/validation_reports/kw/2kwd | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Antibody | Mass: 6228.809 Da / Num. of mol.: 5 / Mutation: T2Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus sp. 'group G' (bacteria) / Gene: spg / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P19909 |
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-Experimental details
-Experiment
Experiment | Method: SOLID-STATE NMR / Details: Crystal Packing of GB1 | ||||||||||||||||
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NMR experiment |
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NMR details | Text: 11.111 kHz MAS |
-Sample preparation
Details |
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Sample |
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Sample conditions | Pressure: 1 atm / Temperature: 273 K |
-NMR measurement
NMR spectrometer | Type: Varian Infinity Plus / Manufacturer: Varian / Model: Infinity Plus / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 600 / Conformers submitted total number: 10 |