+Open data
-Basic information
Entry | Database: PDB / ID: 2krh | ||||||
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Title | Structure of the C-terminal actin binding domain of ABRA | ||||||
Components | Actin-binding Rho-activating protein | ||||||
Keywords | ACTIN-BINDING PROTEIN / muscle / actin binding / stress response / cardiac / Actin-binding / Cytoskeleton | ||||||
Function / homology | Function and homology information positive regulation of Rho protein signal transduction / myofibril / sarcomere / protein import into nucleus / positive regulation of DNA-binding transcription factor activity / actin cytoskeleton / actin binding / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / plasma membrane Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | SOLUTION NMR / torsion angle dynamics, simulated annealing | ||||||
Model details | Solution state NMR structure of the second actin binding domain of ms1/STARS/ABRA. | ||||||
Authors | Fogl, C.L.F. / Pfuhl, M. | ||||||
Citation | Journal: To be Published Title: Structure of the C-terminal actin binding domain of ABRA Authors: Fogl, C.L.F. / Pfuhl, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2krh.cif.gz | 632.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2krh.ent.gz | 560.2 KB | Display | PDB format |
PDBx/mmJSON format | 2krh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kr/2krh ftp://data.pdbj.org/pub/pdb/validation_reports/kr/2krh | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10044.783 Da / Num. of mol.: 1 / Fragment: C-terminal actin binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Abra, Ms1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) / Variant (production host): BL21 STAR (Invitrogen) / References: UniProt: Q8K4K7 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR Details: Solution state NMR structure of the second actin binding domain of ms1/STARS/ABRA. | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.15 / pH: 7.2 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics, simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 3648 / NOE intraresidue total count: 813 / NOE long range total count: 566 / NOE medium range total count: 390 / NOE sequential total count: 1879 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 84 / Protein psi angle constraints total count: 84 | ||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Average torsion angle constraint violation: 0.3669 ° Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 / Maximum lower distance constraint violation: 0.02 Å / Maximum torsion angle constraint violation: 0.4822 ° / Maximum upper distance constraint violation: 0.14 Å / Representative conformer: 1 |