- PDB-2ko6: Solution structure of protein sf3929 from Shigella flexneri 2a. N... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2ko6
Title
Solution structure of protein sf3929 from Shigella flexneri 2a. Northeast Structural Genomics Consortium target SfR81/Ontario Center for Structural Proteomics Target sf3929
Components
Uncharacterized protein yihD
Keywords
Structural Genomics / Unknown function / alpha protein / PSI-2 / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG / Ontario Centre for Structural Proteomics / OCSP
Function / homology
Protein of unknown function DUF1040 / Protein of unknown function DUF1040 / YihD-like superfamily / Protein of unknown function (DUF1040) / Conserved Hypothetical Protein Ylqf; Chain: A; domain 2 / Orthogonal Bundle / Mainly Alpha / Uncharacterized protein / Uncharacterized protein
Mass: 10321.919 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Shigella flexneri (bacteria) / Gene: yihD, S3818, SF3929 / Production host: Escherichia coli (E. coli) / References: UniProt: Q83IV8, UniProt: A0A384LJ77*PLUS
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR Details: Though residue F76-A83 forms a helice, the C-terminal part (residue 61 to residue 89) are in disorder status, no interaction with the core
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
3D HNCO
1
2
1
3DCBCA(CO)NH
1
3
1
3DHBHA(CO)NH
1
4
1
3D HNCA
1
5
1
3DC(CO)NH
1
6
1
3DH(CCO)NH
1
7
1
3D (H)CCH-TOCSY
1
8
1
3D CCH-TOCSY
1
9
1
3D 1H-15N NOESY
1
10
1
3D 1H-13C NOESY
1
11
1
3D 1H-13C NOESY aromatic
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Sample preparation
Details
Contents: 0.5 mM [U-100% 13C; U-100% 15N] sf3929, 10 mM [U-100% 2H] TRIS, 300 mM sodium chloride, 10 uM zinc sulphate, 10 mM [U-100% 2H] DTT, 10 mM benzamidine, 1 x inhibitor cocktail, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O
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