+Open data
-Basic information
Entry | Database: PDB / ID: 2kn0 | ||||||
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Title | Solution NMR Structure of xenopus Fn14 | ||||||
Components | Fn14 | ||||||
Keywords | APOPTOSIS / Fn14 / TWEAK / TNF Receptor / CRD / mutagenesis | ||||||
Function / homology | Function and homology information membrane => GO:0016020 / positive regulation of apoptotic process / plasma membrane Similarity search - Function | ||||||
Biological species | Xenopus laevis (African clawed frog) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | fewest violations, model 1 | ||||||
Authors | Pellegrini, M. / Willen, L. / Perroud, M. / Krushinskie, D. / Strauch, K. / Cuervo, H. / Sun, Y. / Day, E.S. / Schneider, P. / Zheng, T.S. | ||||||
Citation | Journal: Febs J. / Year: 2013 Title: Structure of the extracellular domains of human and Xenopus Fn14: implications in the evolution of TWEAK and Fn14 interactions. Authors: Pellegrini, M. / Willen, L. / Perroud, M. / Krushinskie, D. / Strauch, K. / Cuervo, H. / Day, E.S. / Schneider, P. / Zheng, T.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kn0.cif.gz | 293.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kn0.ent.gz | 252.3 KB | Display | PDB format |
PDBx/mmJSON format | 2kn0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kn/2kn0 ftp://data.pdbj.org/pub/pdb/validation_reports/kn/2kn0 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7697.484 Da / Num. of mol.: 1 / Fragment: residues 23-72 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Xenopus laevis (African clawed frog) / Gene: Fn14, tnfrsf12a / Production host: Pichia Pastoris (fungus) / References: UniProt: Q6SIX7 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: solution NMR structure of xenopus Fn14 CRD |
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NMR experiment | Type: 3D 1H-15N NOESY |
-Sample preparation
Details | Contents: 500-700 uM [U-99% 15N] xeFn14-1, 10 mM sodium phosphate-2, 137 mM sodium chloride-3, 2.7 mM potassium chloride-4, 95% H2O, 5% D2O Solvent system: 95% H2O/5% D2O | ||||||||||||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 140 / pH: 7.4 / Pressure: AMBIENT / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||||||
NMR ensemble | Conformer selection criteria: 20 lowest energy structures / Conformers calculated total number: 100 / Conformers submitted total number: 20 |