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- PDB-2kjk: Solution structure of the second domain of the listeria protein L... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2kjk | ||||||
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Title | Solution structure of the second domain of the listeria protein Lin2157, Northeast Structural Genomics Consortium target Lkr136b | ||||||
![]() | Lin2157 protein | ||||||
![]() | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / PDZ domain / PSI-2 / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG | ||||||
Function / homology | ![]() endopeptidase La / ATP-dependent peptidase activity / protein catabolic process / serine-type endopeptidase activity / proteolysis / ATP binding / membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | fewest violations, model 1 | ||||||
![]() | Wang, X. / Hamilton, K. / Xiao, R.H. / Lee, D. / Ciccosanti, C.H. / Nair, R. / Rost, B. / Acton, T.B. / Swapna, G. / Everett, J.K. ...Wang, X. / Hamilton, K. / Xiao, R.H. / Lee, D. / Ciccosanti, C.H. / Nair, R. / Rost, B. / Acton, T.B. / Swapna, G. / Everett, J.K. / Montelione, G.T. / Prestegard, J.H. / Northeast Structural Genomics Consortium (NESG) | ||||||
![]() | ![]() Title: Solution Structure of Lkr136b Authors: Wang, X. / Montelione, G.T. / Prestegard, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 310.1 KB | Display | ![]() |
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PDB format | ![]() | 256.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 525.6 KB | Display | ![]() |
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Full document | ![]() | 780.5 KB | Display | |
Data in XML | ![]() | 62.4 KB | Display | |
Data in CIF | ![]() | 51.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 11079.544 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.2 / pH: 4.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: structures were first calculated using CYANA's automatic NOE assignment module, which is simulated annealing based. They were then refined in XPLOR-NIH using simulated annealing refinement protocol | |||||||||||||||
NMR representative | Selection criteria: fewest violations | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 50 / Conformers submitted total number: 10 |