Activator of Hsp90 ATPase homologue 1-like / Activator of Hsp90 ATPase homolog 1-like protein / START domain / Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4 / START-like domain superfamily / 2-Layer Sandwich / Alpha Beta / Uncharacterized protein YndB
機能・相同性情報
生物種
Bacillus subtilis subsp. subtilis (枯草菌)
手法
溶液NMR / simulated annealing
Model details
The START domain is found in both eukaryotes and prokaryotes, with putative functions including ...The START domain is found in both eukaryotes and prokaryotes, with putative functions including signal transduction, transcriptional regulation, GTPase activation and thioester hydrolysis. Here we report the near complete 1H, 15N and 13C backbone and side chain NMR resonance assignments for the Bacillus subtilis START domain protein yndB.
Text: The START domain is found in both eukaryotes and prokaryotes, with putative functions including signal transduction, transcriptional regulation, GTPase activation and thioester hydrolysis. Here ...Text: The START domain is found in both eukaryotes and prokaryotes, with putative functions including signal transduction, transcriptional regulation, GTPase activation and thioester hydrolysis. Here we report the near complete 1H, 15N and 13C backbone and side chain NMR resonance assignments for the Bacillus subtilis START domain protein yndB.
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試料調製
詳細
内容: 1 mM [U-100% 13C; U-100% 15N] SR211, 20 mM MES, 5 % D2O, 0.02 % sodium azide, 10 mM DTT, 100 mM sodium chloride, 5 mM calcium chloride, 95% H2O/5% D2O 溶媒系: 95% H2O/5% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
1mM
SR211
[U-100% 13C; U-100% 15N]
1
20mM
MES
1
5 %
D2O
1
0.02 %
sodiumazide
1
10mM
DTT
1
100mM
sodiumchloride
1
5mM
calciumchloride
1
試料状態
イオン強度: 0.105 / pH: 6.5 / 圧: ambient / 温度: 277 K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker Avance
Bruker
AVANCE
600
1
Varian INOVA
Varian
INOVA
750
2
Varian INOVA
Varian
INOVA
600
3
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解析
NMR software
名称
開発者
分類
ARIA
Linge, O'DonoghueandNilges
構造決定
ARIA
Linge, O'DonoghueandNilges
データ解析
ARIA
Linge, O'DonoghueandNilges
精密化
NMRView
Johnson, OneMoonScientific
chemicalshiftassignment
NMRView
Johnson, OneMoonScientific
データ解析
NMRView
Johnson, OneMoonScientific
peakpicking
NMRDraw
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
解析
TALOS
Cornilescu, DelaglioandBax
データ解析
精密化
手法: simulated annealing / ソフトェア番号: 1
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 70 / 登録したコンフォーマーの数: 10