+
データを開く
-
基本情報
登録情報 | データベース: PDB / ID: 2ka2 | ||||||
---|---|---|---|---|---|---|---|
タイトル | Solution NMR structure of BNIP3 transmembrane peptide dimer in detergent micelles with His173-Ser172 intermonomer hydrogen bond restraints | ||||||
![]() | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 | ||||||
![]() | MEMBRANE PROTEIN / integral membrane protein / membrane helix-helix interactions / bnip3 / transmembrane domain / homodimer / membrane protein folding / Apoptosis / Host-virus interaction / Mitochondrion / Phosphoprotein | ||||||
機能・相同性 | ![]() negative regulation of membrane potential / negative regulation of mitochondrial membrane permeability involved in apoptotic process / granzyme-mediated programmed cell death signaling pathway / cellular response to cobalt ion / mitochondrial outer membrane permeabilization / response to oxygen-glucose deprivation / positive regulation of mitochondrial calcium ion concentration / autophagic cell death / negative regulation of mitochondrial fusion / positive regulation of autophagy of mitochondrion ...negative regulation of membrane potential / negative regulation of mitochondrial membrane permeability involved in apoptotic process / granzyme-mediated programmed cell death signaling pathway / cellular response to cobalt ion / mitochondrial outer membrane permeabilization / response to oxygen-glucose deprivation / positive regulation of mitochondrial calcium ion concentration / autophagic cell death / negative regulation of mitochondrial fusion / positive regulation of autophagy of mitochondrion / mitochondrial fragmentation involved in apoptotic process / autophagy of mitochondrion / mitochondrial protein catabolic process / positive regulation of programmed cell death / positive regulation of protein-containing complex disassembly / negative regulation of programmed cell death / intrinsic apoptotic signaling pathway in response to hypoxia / regulation of mitochondrial membrane permeability / regulation of aerobic respiration / positive regulation of mitochondrial fission / positive regulation of cardiac muscle cell apoptotic process / oligodendrocyte differentiation / positive regulation of release of cytochrome c from mitochondria / negative regulation of mitochondrial membrane potential / positive regulation of macroautophagy / negative regulation of reactive oxygen species metabolic process / response to axon injury / response to hyperoxia / positive regulation of autophagy / brown fat cell differentiation / cardiac muscle cell apoptotic process / reactive oxygen species metabolic process / response to bacterium / cellular response to mechanical stimulus / mitochondrial membrane / cerebral cortex development / cellular response to hydrogen peroxide / nuclear envelope / GTPase binding / cellular response to hypoxia / neuron apoptotic process / defense response to virus / mitochondrial outer membrane / postsynaptic density / response to hypoxia / positive regulation of apoptotic process / dendrite / negative regulation of apoptotic process / endoplasmic reticulum / protein homodimerization activity / mitochondrion / nucleoplasm / identical protein binding / nucleus / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
![]() | Sulistijo, E.S. / MacKenzie, K.R. | ||||||
![]() | ![]() タイトル: Structural basis for dimerization of the BNIP3 transmembrane domain 著者: Sulistijo, E.S. / Mackenzie, K.R. | ||||||
履歴 |
|
-
構造の表示
構造ビューア | 分子: ![]() ![]() |
---|
-
ダウンロードとリンク
-
ダウンロード
PDBx/mmCIF形式 | ![]() | 484.2 KB | 表示 | ![]() |
---|---|---|---|---|
PDB形式 | ![]() | 414 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
---|
-関連構造データ
-
リンク
-
集合体
登録構造単位 | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR アンサンブル |
|
-
要素
#1: タンパク質・ペプチド | 分子量: 3790.588 Da / 分子数: 2 / 断片: transmembrane domain, residues 154-188 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
---|
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR実験 |
|
-
試料調製
詳細 | 内容: 0.4 mM [U-100% 13C; U-100% 15N] Bcl2/Adenovirus E1B 19 kDa protein-interacting protein 3, 0.6 mM Bcl2/Adenovirus E1B 19 kDa protein-interacting protein 3, 90% H2O, 10% D2O 溶媒系: 90% H2O/10% D2O | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
試料 |
| ||||||||||||
試料状態 | pH: 5.1 / 温度: 313 K |
-NMR測定
NMRスペクトロメーター |
|
---|
-
解析
NMR software |
| ||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
精密化 | 手法: simulated annealing / ソフトェア番号: 1 詳細: Inferred intermonomer hydrogen bond restraints between His173-Ser172 are included in calculation. Intermonomer hydrogen bond restraints between His173-Ser172 are included. THE CALCULATION OF ...詳細: Inferred intermonomer hydrogen bond restraints between His173-Ser172 are included in calculation. Intermonomer hydrogen bond restraints between His173-Ser172 are included. THE CALCULATION OF THIS STRUCTURE INCLUDES 2 ADDITIONAL HYDROGEN BOND RESTRAINTS THAT WERE DERIVED FROM MUTAGENESIS DATA. | ||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 2036 / NOE intraresidue total count: 1244 / NOE long range total count: 108 / NOE medium range total count: 226 / NOE sequential total count: 386 / Hydrogen bond constraints total count: 58 / Protein chi angle constraints total count: 22 / Protein other angle constraints total count: 8 / Protein phi angle constraints total count: 34 / Protein psi angle constraints total count: 34 | ||||||||||||||||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: lowest energy / 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 20 |