+Open data
-Basic information
Entry | Database: PDB / ID: 2jug | ||||||
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Title | Multienzyme Docking in Hybrid Megasynthetases | ||||||
Components | TubC protein | ||||||
Keywords | BIOSYNTHETIC PROTEIN / docking domain / dimer / nonribosomal peptide synthetase / tubulysin / Ligase / Phosphopantetheine | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Angiococcus disciformis (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Richter, C.D. / Nietlispach, D. / Broadhurst, R.W. / Weissman, K.J. | ||||||
Citation | Journal: Nat.Chem.Biol. / Year: 2008 Title: Multienzyme docking in hybrid megasynthetases Authors: Richter, C.D. / Nietlispach, D. / Broadhurst, R.W. / Weissman, K.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jug.cif.gz | 514.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jug.ent.gz | 439.2 KB | Display | PDB format |
PDBx/mmJSON format | 2jug.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2jug_validation.pdf.gz | 355.5 KB | Display | wwPDB validaton report |
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Full document | 2jug_full_validation.pdf.gz | 474.9 KB | Display | |
Data in XML | 2jug_validation.xml.gz | 29.1 KB | Display | |
Data in CIF | 2jug_validation.cif.gz | 42.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ju/2jug ftp://data.pdbj.org/pub/pdb/validation_reports/ju/2jug | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8126.371 Da / Num. of mol.: 2 / Fragment: sequence database residues 2-74 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Angiococcus disciformis (bacteria) / Genus: Cystobacter / Strain: An d48 / Gene: tubC / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 / Variant (production host): DE3 / References: UniProt: Q5ZPA9 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR Details: Solution structure of the N-terminal docking domain of the TubC subunit of the hybrid non-ribosomal peptide synthetase/polyketide synthase that makes tubulysin | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 0.15 / pH: 6 / Pressure: ambient / Temperature: 308 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 / Details: simulated annealing using Aria 1.2 | ||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 1987 / NOE intraresidue total count: 652 / NOE long range total count: 269 / NOE medium range total count: 449 / NOE sequential total count: 596 / Hydrogen bond constraints total count: 34 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 53 / Protein psi angle constraints total count: 52 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 10 | ||||||||||||||||||||||||||||
NMR ensemble rms | Distance rms dev: 0.049 Å / Distance rms dev error: 0.01 Å |