+Open data
-Basic information
Entry | Database: PDB / ID: 2jss | ||||||
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Title | NMR structure of chaperone Chz1 complexed with histone H2A.Z-H2B | ||||||
Components |
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Keywords | Chaperone/Nuclear Protein / Histone-chaperone complex / intrinsically unfolded protein / Chaperone-Structural Protein COMPLEX / Chaperone-Nuclear Protein COMPLEX | ||||||
Function / homology | Function and homology information HATs acetylate histones / nuclear-transcribed mRNA catabolic process, non-stop decay / Condensation of Prophase Chromosomes / SIRT1 negatively regulates rRNA expression / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / Assembly of the ORC complex at the origin of replication / HDACs deacetylate histones / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / replication fork protection complex / Oxidative Stress Induced Senescence ...HATs acetylate histones / nuclear-transcribed mRNA catabolic process, non-stop decay / Condensation of Prophase Chromosomes / SIRT1 negatively regulates rRNA expression / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / Assembly of the ORC complex at the origin of replication / HDACs deacetylate histones / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / replication fork protection complex / Oxidative Stress Induced Senescence / postreplication repair / silent mating-type cassette heterochromatin formation / RNA Polymerase I Promoter Escape / Estrogen-dependent gene expression / Ub-specific processing proteases / nucleosomal DNA binding / transcription elongation by RNA polymerase II / euchromatin / heterochromatin formation / chromatin DNA binding / structural constituent of chromatin / nucleosome / chromatin organization / histone binding / chromatin remodeling / RNA polymerase II cis-regulatory region sequence-specific DNA binding / protein heterodimerization activity / chromatin binding / regulation of transcription by RNA polymerase II / chromatin / regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / DNA binding / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / torsion angle dynamics, simulated annealing, molecular dynamics | ||||||
Authors | Zhou, Z. / Feng, H. / Hansen, D.F. / Kato, H. / Luk, E. / Freedberg, D.I. / Kay, L.E. / Wu, C. / Bai, Y. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2008 Title: NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B. Authors: Zhou, Z. / Feng, H. / Hansen, D.F. / Kato, H. / Luk, E. / Freedberg, D.I. / Kay, L.E. / Wu, C. / Bai, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jss.cif.gz | 780.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jss.ent.gz | 647.3 KB | Display | PDB format |
PDBx/mmJSON format | 2jss.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2jss_validation.pdf.gz | 356.7 KB | Display | wwPDB validaton report |
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Full document | 2jss_full_validation.pdf.gz | 571.9 KB | Display | |
Data in XML | 2jss_validation.xml.gz | 68.2 KB | Display | |
Data in CIF | 2jss_validation.cif.gz | 91.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/js/2jss ftp://data.pdbj.org/pub/pdb/validation_reports/js/2jss | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 21074.238 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: HTB1, H2B1, SPT12 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-codonplus(DE3)-RIL / References: UniProt: P02293, UniProt: Q12692 |
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#2: Protein | Mass: 6959.446 Da / Num. of mol.: 1 / Fragment: sequence database residues 71-132 / Source method: obtained synthetically / Source: (synth.) Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: P40019 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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