Protein of unknown function DUF3221 / Protein of unknown function (DUF3221) / Nucleic acid-binding proteins / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Nucleic acid-binding, OB-fold / Beta Barrel / Mainly Beta / Uncharacterized protein YobA
Mass: 12528.110 Da / Num. of mol.: 1 / Fragment: sequence database residues 21-120 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: yobA / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)+Magic / References: UniProt: O31835
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
2
2D 1H-13C HSQC
1
3
1
3DCBCA(CO)NH
1
4
1
3D HN(CA)CB
1
5
1
3DHBHA(CO)NH
1
6
1
3D (H)CCH-TOCSY
1
7
1
3D HNHA
1
8
1
3D HNCO
1
9
1
3D CCH-TOCSY
1
10
1
3D 1H-15N NOESY
1
11
1
3D 1H-13C NOESY
1
12
3
4D 1H-13C-13C-1H HMQC-NOESY
-
Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.75 mM [U-100% 13C; U-100% 15N] entity, 10 mM dithiothreitol, 5 mM calcium chloride, 100 mM sodium chloride, 20 mM ammonium acetate, 0.02 % sodium azide, 95% H2O/5% D2O
95% H2O/5% D2O
2
0.79 mM [U-100% N15]; 13C from 5% 13C-glucose in growth medium entity, 10 mM dithiothreitol, 5 mM calcium chloride, 100 mM sodium chloride, 20 mM ammonium acetate, 0.02 % sodium azide, 95% H2O/5% D2O
95% H2O/5% D2O
3
0.75 mM [U-100% 13C; U-100% 15N] entity, 10 mM dithiothreitol, 5 mM calcium chloride, 100 mM sodium chloride, 20 mM ammonium acetate, 0.02 % sodium azide, 100% D2O
100% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
0.75mM
entity
[U-100% 13C; U-100% 15N]
1
10mM
dithiothreitol
1
5mM
calciumchloride
1
100mM
sodiumchloride
1
20mM
ammoniumacetate
1
0.02 %
sodiumazide
1
0.79mM
entity
[U-100% N15]; 13C from 5% 13C-glucose in growth medium
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