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Yorodumi- PDB-2joj: NMR solution structure of N-terminal domain of Euplotes octocarin... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2joj | ||||||
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Title | NMR solution structure of N-terminal domain of Euplotes octocarinatus centrin | ||||||
Components | Centrin protein | ||||||
Keywords | CELL CYCLE / N-terminal domain / centrin solution structure / EF-Hand Calcium Binding Protein | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Euplotes octocarinatus (eukaryote) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Hong, J. / Guo, C. / Lin, D. | ||||||
Citation | Journal: To be Published Title: NMR solution structure of N-terminal domain of Euplotes octocarinatus centrin Authors: Hong, J. / Guo, C. / Lin, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2joj.cif.gz | 439.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2joj.ent.gz | 367.3 KB | Display | PDB format |
PDBx/mmJSON format | 2joj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2joj_validation.pdf.gz | 358 KB | Display | wwPDB validaton report |
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Full document | 2joj_full_validation.pdf.gz | 528.4 KB | Display | |
Data in XML | 2joj_validation.xml.gz | 32.3 KB | Display | |
Data in CIF | 2joj_validation.cif.gz | 48.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jo/2joj ftp://data.pdbj.org/pub/pdb/validation_reports/jo/2joj | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9062.141 Da / Num. of mol.: 1 / Fragment: sequence database residues 21-97 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Euplotes octocarinatus (eukaryote) / Gene: centrin / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): Bl21(de3) / References: UniProt: Q9XZV2 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR |
-Sample preparation
Details |
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Sample |
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Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: In model 16, the link distances for some of the peptide backbone are shorter than average. | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 16 |