+Open data
-Basic information
Entry | Database: PDB / ID: 2j04 | ||||||
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Title | The tau60-tau91 subcomplex of yeast transcription factor IIIC | ||||||
Components |
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Keywords | TRANSCRIPTION / BETA PROPELLER / TYPE 2 PROMOTERS / HYPOTHETICAL PROTEIN / PREINITIATION COMPLEX / YEAST RNA POLYMERASE III / TRANSCRIPTION FACTOR IIIC | ||||||
Function / homology | Function and homology information 5S class rRNA transcription by RNA polymerase III / transcription factor TFIIIC complex / RNA polymerase III general transcription initiation factor activity / RNA Polymerase III Transcription Initiation From Type 2 Promoter / transcription initiation at RNA polymerase III promoter / transcription by RNA polymerase III / DNA binding / nucleus Similarity search - Function | ||||||
Biological species | SACCHAROMYCES CEREVISIAE (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3.2 Å | ||||||
Authors | Mylona, A. / Fernandez-Tornero, C. / Legrand, P. / Muller, C.W. | ||||||
Citation | Journal: Mol.Cell / Year: 2006 Title: Structure of the Tau60/Deltatau91 Subcomplex of Yeast Transcription Factor Iiic: Insights Into Preinitiation Complex Assembly Authors: Mylona, A. / Fernandez-Tornero, C. / Legrand, P. / Haupt, M. / Sentenac, A. / Acker, J. / Muller, C.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2j04.cif.gz | 401.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2j04.ent.gz | 329.8 KB | Display | PDB format |
PDBx/mmJSON format | 2j04.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2j04_validation.pdf.gz | 478 KB | Display | wwPDB validaton report |
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Full document | 2j04_full_validation.pdf.gz | 604.4 KB | Display | |
Data in XML | 2j04_validation.xml.gz | 80.5 KB | Display | |
Data in CIF | 2j04_validation.cif.gz | 108.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j0/2j04 ftp://data.pdbj.org/pub/pdb/validation_reports/j0/2j04 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
NCS oper:
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-Components
#1: Protein | Mass: 67755.172 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Plasmid: PVL1393 / Cell line (production host): High Five / Production host: SPODOPTERA FRUGIPERDA (fall armyworm) / References: UniProt: Q12308 #2: Protein | Mass: 58928.871 Da / Num. of mol.: 2 / Fragment: PROPELLER DOMAIN, RESIDUES 159-672 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Plasmid: PET-28A / Production host: ESCHERICHIA COLI BL21(DE3) (bacteria) / References: UniProt: Q06339 #3: Water | ChemComp-HOH / | Sequence details | N-TERMINAL CLONING ARTIFACT C-TERMINAL NON-CLEVABLE HIS- TAG | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.42 Å3/Da / Density % sol: 63.74 % |
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Crystal grow | pH: 7 / Details: 0.8 M AMMONIUM SULFATE, 0.1 M HEPES PH 7.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.9793 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Dec 15, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→30 Å / Num. obs: 49476 / % possible obs: 98.6 % / Observed criterion σ(I): 1.8 / Redundancy: 3.2 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 5.4 |
Reflection shell | Resolution: 3.2→3.4 Å / Redundancy: 3 % / Rmerge(I) obs: 0.4 / Mean I/σ(I) obs: 1.8 / % possible all: 97.9 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 3.2→208.51 Å / Cor.coef. Fo:Fc: 0.926 / Cor.coef. Fo:Fc free: 0.882 / SU B: 54.87 / SU ML: 0.409 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R Free: 0.491 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 70.92 Å2
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Refinement step | Cycle: LAST / Resolution: 3.2→208.51 Å
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Refine LS restraints |
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