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Yorodumi- PDB-2imm: Refined crystal structure of a recombinant immunoglobulin domain ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2imm | ||||||
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Title | Refined crystal structure of a recombinant immunoglobulin domain and a complementarity-determining region 1-grafted mutant | ||||||
Components | IGA-KAPPA MCPC603 FV (LIGHT CHAIN) | ||||||
Keywords | IMMUNOGLOBULIN | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / ACETATE ION / : Function and homology information | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Steipe, B. / Huber, R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1992 Title: Refined crystal structure of a recombinant immunoglobulin domain and a complementarity-determining region 1-grafted mutant. Authors: Steipe, B. / Pluckthun, A. / Huber, R. #1: Journal: J.Mol.Biol. / Year: 1990 Title: Crystallization and Preliminary X-Ray Studies of the VL Domain of the Antibody Mc/Pc603 Produced in Escherichia Coli Authors: Glockshuber, R. / Steipe, B. / Huber, R. / Pluckthun, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2imm.cif.gz | 37.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2imm.ent.gz | 25.3 KB | Display | PDB format |
PDBx/mmJSON format | 2imm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2imm_validation.pdf.gz | 386.4 KB | Display | wwPDB validaton report |
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Full document | 2imm_full_validation.pdf.gz | 386.6 KB | Display | |
Data in XML | 2imm_validation.xml.gz | 3.8 KB | Display | |
Data in CIF | 2imm_validation.cif.gz | 6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/im/2imm ftp://data.pdbj.org/pub/pdb/validation_reports/im/2imm | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Atom site foot note | 1: RESIDUES PRO 8 AND PRO 95 ARE CIS-PROLINES. 2: RESIDUES LYS 103 AND ARG 108 ARE PARTIALLY DISORDERED IN THE ELECTRON DENSITY. | ||||||||
Components on special symmetry positions |
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-Components
#1: Antibody | Mass: 12341.727 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / References: GenBank: 208622 |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-ACT / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.26 Å3/Da / Density % sol: 62.3 % | |||||||||||||||
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Crystal grow | *PLUS pH: 4 / Method: unknown | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 1.9 Å / Num. obs: 11262 / Num. measured all: 96963 / Rmerge(I) obs: 0.086 |
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-Processing
Software | Name: EREF / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Rfactor Rwork: 0.149 / Highest resolution: 2 Å Details: RESIDUES LYS 103 AND ARG 108 ARE PARTIALLY DISORDERED IN THE ELECTRON DENSITY | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2 Å / Rfactor obs: 0.149 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: o_angle_d / Dev ideal: 2.23 |