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- PDB-2ihx: Solution Structure of the Rous Sarcoma Virus Nucleocapsid Protein... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2ihx | ||||||
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Title | Solution Structure of the Rous Sarcoma Virus Nucleocapsid Protein:uPsi RNA Packaging Signal Complex | ||||||
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![]() | VIRAL PROTEIN/RNA / Protein-RNA complex / VIRAL PROTEIN-RNA COMPLEX | ||||||
Function / homology | ![]() host cell nucleoplasm / viral procapsid maturation / virion component => GO:0044423 / host cell nucleolus / Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / viral process / viral capsid / nucleic acid binding / structural constituent of virion / aspartic-type endopeptidase activity ...host cell nucleoplasm / viral procapsid maturation / virion component => GO:0044423 / host cell nucleolus / Hydrolases; Acting on peptide bonds (peptidases); Aspartic endopeptidases / viral process / viral capsid / nucleic acid binding / structural constituent of virion / aspartic-type endopeptidase activity / host cell plasma membrane / proteolysis / zinc ion binding / membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / 1H-1H distance restraints, Hydrogen-bond restraints, Torsion angle restraints, Inter-phosphate restraints | ||||||
![]() | Zhou, J. / Summers, M. | ||||||
![]() | ![]() Title: Solution Structure of the Rous Sarcoma Virus Nucleocapsid Protein: muPsi RNA Packaging Signal Complex. Authors: Zhou, J. / Bean, R.L. / Vogt, V.M. / Summers, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.4 MB | Display | ![]() |
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PDB format | ![]() | 1.2 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 388.4 KB | Display | ![]() |
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Full document | ![]() | 676.9 KB | Display | |
Data in XML | ![]() | 84 KB | Display | |
Data in CIF | ![]() | 116.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 24267.391 Da / Num. of mol.: 1 Fragment: minimal RNA packaging signal in the 5'- untranslated region (UTR) of Rous sarcoma virus (RSV) Source method: obtained synthetically Details: RNA was prepared by in vitro T7 RNA transcription. The sequence occurs naturally in Rous sarcoma virus (RSV) |
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#2: Protein | Mass: 6706.681 Da / Num. of mol.: 1 / Fragment: Nucleocapsid domain (residues 503-563) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample conditions | Ionic strength: 5 mM NaCl, 0.1 mM ZnCl2 / pH: 7.0 / Pressure: ambient / Temperature: 308 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: 1H-1H distance restraints, Hydrogen-bond restraints, Torsion angle restraints, Inter-phosphate restraints Software ordinal: 1 Details: The structures are based on a total of 1697 restraints. 680 are NOE-derived distance restraints, 608 are Hydrogen-bond restraints, 239 are torsion angle restraints and 170 are inter-phosphate restraints. | ||||||||||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 800 / Conformers submitted total number: 20 |