death receptor activity / neuron projection membrane / cell body membrane / neurotrophin p75 receptor binding / nerve growth factor binding / nuclear envelope lumen / Rho protein signal transduction / coreceptor activity / lamellipodium / apoptotic process ...death receptor activity / neuron projection membrane / cell body membrane / neurotrophin p75 receptor binding / nerve growth factor binding / nuclear envelope lumen / Rho protein signal transduction / coreceptor activity / lamellipodium / apoptotic process / cell surface / membrane / nucleus / plasma membrane Similarity search - Function
Tumor necrosis factor receptor member 16, transmembrane domain / Tumor necrosis factor receptor member 16 trans-membrane domain / Death Domain, Fas / Death Domain, Fas / Death domain profile. / DEATH domain, found in proteins involved in cell death (apoptosis). / Death domain / Death domain / Death-like domain superfamily / Orthogonal Bundle / Mainly Alpha Similarity search - Domain/homology
DeathdomaincontainingmembraneproteinNRADD / Neurotrophin receptor associated death domain
Mass: 9975.160 Da / Num. of mol.: 1 / Fragment: residues 138-228 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Nradd / Plasmid: pGEX2T / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q8CJ26
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
HNCA-J
1
2
1
3D 15N-separated NOESY
1
3
1
3D 13C-separated NOESY
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Sample preparation
Details
Contents: 1-2 mM protein, PBS (Phosphate Buffer Saline) buffer, pH 7.5, 298 K Solvent system: PBS (Phosphate Buffer Saline) buffer, pH 7.5, 298 K
Sample conditions
Ionic strength: 137 mM NaCl / pH: 7.5 / Pressure: ambient / Temperature: 298 K
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NMR measurement
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelength
Relative weight: 1
NMR spectrometer
Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 750 MHz
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Processing
NMR software
Name
Developer
Classification
CYANA
Guntert
structuresolution
CNS
refinement
XwinNMR
collection
Refinement
Method: simulated annealing / Software ordinal: 1 Details: the structures are based on a total of 1319 restraints, 1053 are NOE-derived distance constraints, 266 dihedral angle restraints.
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20
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