ソフトウェア | 名称 | バージョン | 分類 |
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REFMAC | 5.2.0019精密化 | HKL-2000 | | データ削減 | HKL-2000 | | データスケーリング | MOLREP | | 位相決定 | |
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精密化 | 構造決定の手法: 分子置換 / 解像度: 2.67→70.71 Å / Cor.coef. Fo:Fc: 0.944 / Cor.coef. Fo:Fc free: 0.879 / SU B: 35.443 / SU ML: 0.35 / TLS residual ADP flag: LIKELY RESIDUAL / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R Free: 0.44 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
| Rfactor | 反射数 | %反射 | Selection details |
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Rfree | 0.29841 | 510 | 4.8 % | RANDOM |
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Rwork | 0.20011 | - | - | - |
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obs | 0.2048 | 10107 | 98.77 % | - |
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all | - | 10107 | - | - |
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK |
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原子変位パラメータ | Biso mean: 29.419 Å2
| Baniso -1 | Baniso -2 | Baniso -3 |
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1- | 2.51 Å2 | 0 Å2 | 1.77 Å2 |
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2- | - | 0.61 Å2 | 0 Å2 |
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3- | - | - | -1.18 Å2 |
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精密化ステップ | サイクル: LAST / 解像度: 2.67→70.71 Å
| タンパク質 | 核酸 | リガンド | 溶媒 | 全体 |
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原子数 | 2876 | 0 | 0 | 41 | 2917 |
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拘束条件 | Refine-ID | タイプ | Dev ideal | Dev ideal target | 数 |
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X-RAY DIFFRACTION | r_bond_refined_d0.011 | 0.022 | 2936 | X-RAY DIFFRACTION | r_bond_other_d | | | X-RAY DIFFRACTION | r_angle_refined_deg1.313 | 1.978 | 3968 | X-RAY DIFFRACTION | r_angle_other_deg | | | X-RAY DIFFRACTION | r_dihedral_angle_1_deg6.649 | 5 | 362 | X-RAY DIFFRACTION | r_dihedral_angle_2_deg36.311 | 24.167 | 120 | X-RAY DIFFRACTION | r_dihedral_angle_3_deg18.665 | 15 | 536 | X-RAY DIFFRACTION | r_dihedral_angle_4_deg16.937 | 15 | 14 | X-RAY DIFFRACTION | r_chiral_restr0.086 | 0.2 | 456 | X-RAY DIFFRACTION | r_gen_planes_refined0.004 | 0.02 | 2150 | X-RAY DIFFRACTION | r_gen_planes_other | | | X-RAY DIFFRACTION | r_nbd_refined0.227 | 0.2 | 1420 | X-RAY DIFFRACTION | r_nbd_other | | | X-RAY DIFFRACTION | r_nbtor_refined0.307 | 0.2 | 2053 | X-RAY DIFFRACTION | r_nbtor_other | | | X-RAY DIFFRACTION | r_xyhbond_nbd_refined0.171 | 0.2 | 112 | X-RAY DIFFRACTION | r_xyhbond_nbd_other | | | X-RAY DIFFRACTION | r_metal_ion_refined | | | X-RAY DIFFRACTION | r_metal_ion_other | | | X-RAY DIFFRACTION | r_symmetry_vdw_refined0.29 | 0.2 | 40 | X-RAY DIFFRACTION | r_symmetry_vdw_other | | | X-RAY DIFFRACTION | r_symmetry_hbond_refined0.3 | 0.2 | 3 | X-RAY DIFFRACTION | r_symmetry_hbond_other | | | X-RAY DIFFRACTION | r_symmetry_metal_ion_refined | | | X-RAY DIFFRACTION | r_symmetry_metal_ion_other | | | X-RAY DIFFRACTION | r_mcbond_it0.436 | 1.5 | 1857 | X-RAY DIFFRACTION | r_mcbond_other | | | X-RAY DIFFRACTION | r_mcangle_it0.767 | 2 | 2954 | X-RAY DIFFRACTION | r_scbond_it0.956 | 3 | 1199 | X-RAY DIFFRACTION | r_scangle_it1.562 | 4.5 | 1014 | X-RAY DIFFRACTION | r_rigid_bond_restr | | | X-RAY DIFFRACTION | r_sphericity_free | | | X-RAY DIFFRACTION | r_sphericity_bonded | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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LS精密化 シェル | 解像度: 2.67→2.742 Å / Total num. of bins used: 20
| Rfactor | 反射数 | %反射 |
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Rfree | 0.381 | 37 | - |
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Rwork | 0.309 | 659 | - |
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obs | - | - | 89.12 % |
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精密化 TLS | 手法: refined / Refine-ID: X-RAY DIFFRACTION ID | L11 (°2) | L12 (°2) | L13 (°2) | L22 (°2) | L23 (°2) | L33 (°2) | S11 (Å °) | S12 (Å °) | S13 (Å °) | S21 (Å °) | S22 (Å °) | S23 (Å °) | S31 (Å °) | S32 (Å °) | S33 (Å °) | T11 (Å2) | T12 (Å2) | T13 (Å2) | T22 (Å2) | T23 (Å2) | T33 (Å2) | Origin x (Å) | Origin y (Å) | Origin z (Å) |
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1 | 4.7088 | -4.4663 | -9.5679 | 4.2363 | 9.0752 | 19.4414 | -0.1086 | -0.4245 | 0.4611 | 0.6037 | 0.5438 | 0.0249 | -0.5488 | 0.2531 | -0.4353 | 0.3251 | -0.049 | 0.0096 | 0.3174 | 0.013 | 0.2782 | 15.3454 | 12.4199 | 28.1342 | 2 | 7.8378 | 6.5479 | 2.7063 | 7.6671 | 0.0587 | 6.2053 | -0.2626 | 0.249 | 0.1501 | -1.1897 | -0.3027 | 0.1817 | -0.2618 | -0.5599 | 0.5654 | 0.1828 | 0.1077 | -0.0699 | 0.2696 | -0.0664 | 0.4211 | 1.9993 | 4.0414 | 16.0815 | 3 | 4.6218 | -3.496 | 6.1829 | 9.7915 | 4.9248 | 21.1708 | 0.0209 | -0.0398 | 0.1967 | -0.0225 | -0.8946 | 0.3668 | 0.1728 | -1.2735 | 0.8736 | 0.0924 | 0.0296 | 0.0593 | 0.2796 | -0.0175 | 0.4324 | -3.6376 | 4.4187 | 25.6551 | 4 | 0.8792 | 2.1018 | 0.3269 | 5.0246 | 0.7814 | 0.1215 | -0.2526 | -1.0061 | 0.3101 | 0.3621 | 0.3062 | 0.0143 | -0.2932 | -0.258 | -0.0536 | 0.3132 | 0.0556 | 0.0632 | 0.3831 | 0.0091 | 0.2769 | 12.4625 | 5.7884 | 34.4135 | 5 | 37.5036 | 3.9441 | 7.1222 | 42.5557 | 9.0325 | 2.9808 | 0.9197 | -0.3322 | -3.2225 | 0.6108 | 0.3166 | -1.4836 | 0.6651 | -0.1392 | -1.2363 | 0.3143 | 0.1755 | 0.0467 | 0.4314 | 0.0237 | 0.3336 | 14.4242 | -7.8747 | 36.5626 | 6 | 4.8338 | 2.0779 | -7.2021 | 28.9451 | -19.445 | 33.0645 | -0.0421 | -1.2024 | 0.0802 | 0.2411 | 0.2842 | -2.0738 | 0.6894 | 0.3634 | -0.2421 | 0.1762 | 0.0562 | -0.0964 | 0.6086 | -0.0883 | 0.1494 | 24.6987 | 1.8913 | 38.6191 | 7 | 8.0984 | -0.5385 | -3.4292 | 4.1787 | 0.0807 | 6.8258 | -0.1569 | -0.6257 | 0.3522 | 0.2013 | 0.2468 | 0.2031 | -0.1112 | 0.345 | -0.0899 | 0.3019 | 0.0446 | -0.0106 | 0.2552 | -0.0111 | 0.2324 | 15.473 | 5.0475 | 29.8022 | 8 | 2.2419 | 0.7449 | 0.8673 | 0.2475 | 0.2882 | 0.3356 | -0.1819 | 0.3317 | -0.3888 | 0.0165 | -0.0895 | -0.0096 | 0.0168 | -0.1191 | 0.2714 | 0.3524 | 0.0071 | 0.0313 | 0.3223 | 0.0665 | 0.4079 | 20.3761 | -0.415 | 16.7382 | 9 | 4.7267 | -1.5494 | -5.2118 | 1.5377 | 3.7706 | 9.8764 | -0.0517 | 0.2468 | 0.1302 | -0.4296 | 0.3676 | 0.2875 | -0.2505 | -0.3645 | -0.3159 | 0.2977 | 0.0551 | -0.0031 | 0.3668 | 0.0702 | 0.2525 | 22.1738 | -1.7019 | 9.2074 | 10 | 8.512 | 3.5301 | 6.8085 | 2.5231 | 2.7245 | 5.4551 | -1.5617 | 0.0089 | -0.4743 | 0.5537 | 0.5349 | -0.1252 | 0.994 | -1.0408 | 1.0268 | 0.5488 | 0.1468 | 0.0367 | 0.2389 | 0.0386 | 0.2323 | 9.1493 | -6.2898 | 31.6403 | 11 | 33.2987 | 0.736 | -19.6031 | 0.4444 | 2.3384 | 29.4832 | -0.3672 | -0.1237 | -0.9793 | 0.7406 | 0.265 | 0.2252 | 1.5321 | -0.4861 | 0.1022 | 0.3537 | -0.0062 | 0.105 | 0.1469 | 0.0215 | 0.5814 | 0.3046 | -4.3496 | 28.5135 | 12 | 4.2493 | 0.4062 | -2.3657 | 4.6721 | 1.8598 | 3.173 | 0.0659 | 0.0959 | 0.0051 | -0.2966 | -0.1436 | 0.0141 | -0.2346 | 0.1712 | 0.0777 | 0.2703 | 0.0135 | -0.0645 | 0.3447 | 0.038 | 0.2557 | 11.9967 | -1.5649 | 14.6809 | 13 | 4.732 | 1.1508 | -0.3713 | 2.842 | -3.0616 | 7.1426 | 0.0304 | -0.4023 | -0.5899 | -0.1573 | 0.0483 | 1.2408 | 0.6048 | -0.4921 | -0.0786 | 0.2465 | -0.0135 | 0.0534 | 0.3664 | -0.0757 | 0.4072 | 6.0321 | -13.2451 | 17.7572 | 14 | 20.1543 | -4.0401 | 6.0467 | 5.3013 | -1.5022 | 2.9138 | -0.3435 | 0.0399 | -0.6611 | -0.1015 | 0.0817 | 0.6487 | 0.199 | -0.0382 | 0.2618 | 0.3556 | -0.0434 | 0.0724 | 0.2615 | -0.0281 | 0.1963 | 20.6271 | -18.1567 | 15.2383 | 15 | 19.9954 | 2.8494 | 13.2944 | 13.2526 | 9.5539 | 13.4058 | 2.9354 | -1.1238 | -1.3262 | 4.6254 | -0.3431 | 2.9512 | 1.7249 | -1.4476 | -2.5923 | 0.9502 | 0.0781 | 0.2098 | 0.4299 | 0.2952 | 0.4446 | 28.6319 | -17.3449 | 29.8855 | 16 | 7.6759 | -0.1392 | -3.3758 | 9.9878 | -8.4596 | 16.5132 | 0.1281 | 0.7525 | 0.8135 | 0.2037 | -0.594 | -1.0593 | -0.7363 | 0.5752 | 0.4658 | 0.1707 | 0.0243 | 0.0101 | 0.3187 | -0.0237 | 0.3281 | 42.7392 | 2.5927 | 12.8824 | 17 | 5.0901 | 0.0404 | -2.1304 | 33.8781 | 1.0758 | 4.081 | 0.2497 | -0.7255 | -0.2646 | 0.397 | -0.2957 | -1.2241 | 0.2573 | 0.6168 | 0.046 | 0.1513 | 0.1007 | -0.0355 | 0.403 | -0.0113 | 0.2841 | 43.904 | -14.5871 | 21.0908 | 18 | 0.9264 | -1.7053 | 0.0312 | 4.2884 | -1.7695 | 2.5519 | -0.4204 | 0.038 | -0.0731 | -0.2712 | 0.1516 | -0.8315 | 0.417 | 0.5122 | 0.2688 | 0.2462 | 0.1062 | 0.0343 | 0.3733 | -0.0012 | 0.4098 | 48.0735 | -18.3285 | 10.4203 | 19 | 2.1497 | -1.0727 | -1.1834 | 4.4943 | -2.7194 | 3.4187 | -0.0013 | 0.2332 | 0.1082 | -0.3132 | 0.0933 | -0.4149 | -0.209 | 0.4609 | -0.092 | 0.2153 | -0.0204 | 0.0747 | 0.3606 | -0.0747 | 0.2874 | 38.7956 | -3.0125 | 3.126 | 20 | 2.0302 | -2.4905 | 1.7473 | 7.0734 | 2.5181 | 9.727 | -0.1956 | 0.7359 | 0.747 | -0.8152 | -0.012 | 0.6719 | -0.7487 | -0.3277 | 0.2076 | 0.3476 | 0.057 | 0.0635 | 0.3657 | 0.1784 | 0.2873 | 28.5757 | 2.631 | 0.9332 | 21 | 6.5621 | -0.6152 | -3.539 | 4.7944 | -1.3279 | 11.6026 | -0.3529 | 0.0325 | 0.7698 | 0.4637 | 0.2562 | -0.2089 | -0.723 | -0.2312 | 0.0967 | 0.2378 | 0.0144 | -0.0715 | 0.2841 | -0.0154 | 0.3046 | 33.2674 | 2.7474 | 14.2881 | 22 | 2.6155 | -1.8796 | -1.4445 | 4.025 | 1.1078 | 3.2157 | -0.1599 | 0.314 | 0.6165 | -0.9112 | 0.2842 | -0.6246 | 0.2513 | 0.514 | -0.1242 | 0.3074 | 0.0288 | 0.0474 | 0.4135 | -0.0283 | 0.2947 | 41.0783 | -7.1411 | 4.6016 | 23 | 24.055 | -0.3822 | -3.6941 | 0.0061 | 0.0587 | 0.5673 | -0.3879 | -0.4149 | -1.9654 | 0.2526 | -0.0429 | 0.3719 | -0.0103 | 0.9557 | 0.4308 | 0.4588 | 0.1712 | 0.066 | 0.4295 | 0.0517 | 0.2556 | 24.3121 | -3.3434 | 27.3713 | 24 | 5.9297 | -2.8602 | -2.4643 | 10.4513 | 11.817 | 21.4409 | -0.4536 | -0.8018 | 0.122 | 1.0387 | 0.3394 | 0.0364 | 0.6521 | 0.3221 | 0.1142 | 0.3414 | 0.0912 | -0.0359 | 0.3065 | 0.0621 | 0.271 | 26.1008 | -7.1661 | 27.6361 | 25 | 6.3395 | -2.4645 | -9.2731 | 7.0868 | 1.9913 | 23.0357 | 0.1016 | 0.6126 | -0.1435 | -0.9389 | 0.2412 | -0.6065 | -0.1844 | 0.1727 | -0.3428 | 0.3423 | -0.0854 | 0.0479 | 0.3777 | -0.0758 | 0.2378 | 33.8438 | -12.35 | 4.1133 | 26 | 9.9565 | -3.0559 | -0.2955 | 6.3117 | -1.5356 | 0.501 | -0.0607 | 1.0087 | -0.7608 | -0.6909 | -0.1459 | 0.2399 | 0.1979 | 0.068 | 0.2066 | 0.3158 | 0.1176 | 0.0065 | 0.3392 | -0.0701 | 0.3903 | 39.5563 | -17.7147 | 7.9949 | 27 | 2.6346 | -0.8523 | 0.2806 | 3.6961 | 1.785 | 4.2118 | -0.3591 | -0.5392 | -0.0913 | 0.3139 | 0.1667 | -0.3216 | 0.2599 | -0.0477 | 0.1924 | 0.3387 | 0.0631 | 0.0089 | 0.3443 | 0.0126 | 0.2617 | 31.1823 | -12.9413 | 20.7389 | 28 | 1.0671 | -1.1126 | -3.1986 | 2.4282 | 2.5973 | 10.0174 | 0.0489 | 0.1756 | -0.5878 | 0.3626 | 0.3103 | 0.0408 | 0.9495 | 0.2962 | -0.3592 | 0.2889 | -0.0063 | 0.0071 | 0.3064 | -0.0623 | 0.3152 | 29.935 | -22.9565 | 12.8387 | 29 | 12.4479 | -4.0044 | 4.557 | 2.3174 | -0.6433 | 2.3257 | -0.3732 | 0.206 | -0.7794 | 0.2597 | 0.2028 | 0.3849 | 0.068 | -0.1233 | 0.1704 | 0.2607 | 0.0023 | 0.1069 | 0.3502 | 0.0236 | 0.236 | 17.5402 | -16.7942 | 16.9587 | 30 | 17.8434 | 2.9454 | 24.6382 | 1.1569 | -0.2167 | 61.3794 | -0.5828 | 2.6198 | -1.0379 | -3.4599 | 1.2537 | 0.0546 | 0.1935 | 1.469 | -0.6709 | 0.7627 | 0.0125 | -0.0275 | 0.2881 | -0.0681 | 0.3025 | 12.7168 | -4.0703 | 3.7936 |
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精密化 TLSグループ | ID | Refine-ID | Refine TLS-ID | Auth asym-ID | Label asym-ID | Auth seq-ID | Label seq-ID |
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1 | X-RAY DIFFRACTION | 1 | AA3 - 12 | 3 - 12 | 2 | X-RAY DIFFRACTION | 2 | AA13 - 26 | 13 - 26 | 3 | X-RAY DIFFRACTION | 3 | AA27 - 38 | 27 - 38 | 4 | X-RAY DIFFRACTION | 4 | AA39 - 54 | 39 - 54 | 5 | X-RAY DIFFRACTION | 5 | AA55 - 62 | 55 - 62 | 6 | X-RAY DIFFRACTION | 6 | AA63 - 72 | 63 - 72 | 7 | X-RAY DIFFRACTION | 7 | AA73 - 100 | 73 - 100 | 8 | X-RAY DIFFRACTION | 8 | AA101 - 108 | 101 - 108 | 9 | X-RAY DIFFRACTION | 9 | AA109 - 119 | 109 - 119 | 10 | X-RAY DIFFRACTION | 10 | AA120 - 127 | 120 - 127 | 11 | X-RAY DIFFRACTION | 11 | AA128 - 136 | 128 - 136 | 12 | X-RAY DIFFRACTION | 12 | AA137 - 157 | 137 - 157 | 13 | X-RAY DIFFRACTION | 13 | AA158 - 171 | 158 - 171 | 14 | X-RAY DIFFRACTION | 14 | AA172 - 180 | 172 - 180 | 15 | X-RAY DIFFRACTION | 15 | AA181 - 184 | 181 - 184 | 16 | X-RAY DIFFRACTION | 16 | BB3 - 12 | 3 - 12 | 17 | X-RAY DIFFRACTION | 17 | BB13 - 24 | 13 - 24 | 18 | X-RAY DIFFRACTION | 18 | BB25 - 38 | 25 - 38 | 19 | X-RAY DIFFRACTION | 19 | BB39 - 59 | 39 - 59 | 20 | X-RAY DIFFRACTION | 20 | BB60 - 72 | 60 - 72 | 21 | X-RAY DIFFRACTION | 21 | BB73 - 88 | 73 - 88 | 22 | X-RAY DIFFRACTION | 22 | BB89 - 104 | 89 - 104 | 23 | X-RAY DIFFRACTION | 23 | BB105 - 110 | 105 - 110 | 24 | X-RAY DIFFRACTION | 24 | BB111 - 118 | 111 - 118 | 25 | X-RAY DIFFRACTION | 25 | BB119 - 128 | 119 - 128 | 26 | X-RAY DIFFRACTION | 26 | BB129 - 139 | 129 - 139 | 27 | X-RAY DIFFRACTION | 27 | BB140 - 159 | 140 - 159 | 28 | X-RAY DIFFRACTION | 28 | BB160 - 169 | 160 - 169 | 29 | X-RAY DIFFRACTION | 29 | BB170 - 180 | 170 - 180 | 30 | X-RAY DIFFRACTION | 30 | BB181 - 184 | 181 - 184 | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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